Effect On The Fungal Diversity From Rhizosphere Soil Of Suaeda Liaotungensis By Season And Salinity Variation

Liaoning Province is one of the major Suaeda habitat in China. But it is still remain unknown on the population structure and distribution characteristics of fungal species in rhizosphere soil of Suaeda liaotungensis. In present paper, the fungal species from rhizosphere soil of Suaeda liaotungensis by soil dilution-plate and the traditional morphological methods was isolated and identified. Effect of the fungal diversity from rhizosphere soil by seasonal variation and salinity were analysed. The experimental results showed that there were 6410, 4180, 5730 and 3340 fungal colonies in rhizosphere soil in spring, summer, autumn and winter, respectively and they belong to 6 genus 13 species, 9 genus 16 species, 11 genus 31 species and 6 genus 12 species respectively and 13 genera 42 species fungi in Suaeda liaotungensis rhizosphere soil were isolated and identified, of which the species in Penicillium, Stachybotrys, Cladosporium, Trichoderma, Aspergillus and Fusarium were the dominant groups of fungi. The fungal population structures and compositions were different in Suaeda liaotungensis rhizosphere soil. In different seasons.The Shannon diversity index, richness index and evenness index of rhizosphere fungi from different seasons were autumn>summer>spring>winter. Shannon diversity index, richness index and evenness index were the highest in autumn. The richness of fungal species in rhizosphere soil of Suaeda liaotungensis was nearly seven times than that in the unvegetated soil. The Shannon diversity index and evenness index of rhizosphere fungi was almost 4~8 and 1~1.2 times as high as the unvegetated soil, respectively. The salt stress habitats was constructed by NaCl concentration gradient method for effecting diversity of fungal species in rhizosphere soil of Suaeda liaotungensis, the result showed that about 60% of fungi could tolerate media with less than 5% NaCl, and nearly 15% fungus were able to grow in media with higher than 10% NaCl. Aspergillus fumigatus and Alternaria tenuissima were tolerant to 16% NaCl or higher. Aspergillus Ochraceus, the most salt-tolerant fungi was tolerant to 20% NaCl. Our results demonstrated that the factors such as climate and living environment evidently affected the fungal diversity and similarity in the rhizosphere soil of Suaeda liaotungensis.Eight kinds of Alternaria alternata antagonistic microorganisms were obtained by plate confrontation. Three kinds of them have obvious effects, the best effect of them was Aspergillus fumigatus 、 Aspergiltus Ochratoxin and Penicillium oxalicum. In addition, four kinds of Colletotrichum gloeosporioides antagonistic microorganisms were screened, but among them only Aspergiltus Ochratoxin have obvious effect.The innovation of this paper is to study the effect on the fungal diversity from rhizosphere soil of Suaeda liaotungensis by change of salinity and season, it has an important significance for further studying Suaeda fungi diversity. Therefore, The aims of the paper were to analyze the fungal diversity in rhizosphere soil of Suaeda liaotungensis in different seasons,to analyze the influence of salinity on the fungal diversity in rhizosphere soil of Suaeda liaotungensis, to collect potentially economic fungal resources and to understand Suaeda liaotungensis protective effect on soil fungi.