Establishment Of Rapid Propagation System And Study Of Radiation Mutagenesis Of Gardenia

The gardenia leaves were used as the explant in this experiment. Successfully, we set up the system of rapid propagation of gardenia through different hormone combinations. The optimum culture medium of Gardenia callus is MS+2.0mg/L2,4-D+0.02mg/L6-BA, the induction ratio of gardenia callus reaches100%; The optimum culture medium having the92%induction ratio of adventitious bud induction is MS+0.5mg/L6-BA+0.2mg/L NAA; The optimum culture medium with96.7%induction ratio is1/2MS+0.5mg/L NAA, which could be used as the optimum culture medium for roots.We found the obvious effect of regenerative abilities after the gardenia callus processed by different doses of60Co-γ. In20Gy, survival ratio, bud ratio and rooting ratio of gardenia callus respectively reached67%,61.11%,57.15%. In40Gy, survival ratio, bud ratio and rooting ratio of gardenia callus respectively reached53%,37.97%,43.76%. In50Gy, survival ratio, bud ratio and rooting ratio of gardenia callus respectively reached21%,14.40%,48.33%. The gardenia callus could not survive in60Gy. The half lethal dose is40Gy and lethal dose is60Gy.The obvious mutation of chromosome of gardenia callus in different doses of60Co-y could be found easily. The mutation ratio which was the highest ratio reached2.5%in40Gy; the mutation ratio in50Gy is2.1%and in20Gy is1.7%; Respectively, the ratio of chromosome bridge, fragment and multinuclear reached3.25%,2.0%,0.75%. The ratio of micronucleus is0.7%.In order to detecting plant genetic material and effect of different doses of radiation in the molecular level, we used Random Amplified Polymorphism DNA (RAPD) to screen three variation materials and control group. We selected5primers from26random primers, showing obvious polymorphisms and gaining48bands. The results showed that different doses of radiation could gain gardenia polymorphisms, having differences in DNA level.