The Correlation Between Mycotoxins And Ergosterol In Alfalfa Hay

The experiment is designed to use ergosterol content to estimate mycotoxins content in alfalfa hay, thus reflecting the degree of mycotoxins infection. In view of the above research aim, the study has been carried on the following work: Established the methods of mycotoxins (AFs, ZEN, DON, OTA, FBs, T-2) and researched the correlation between mycotoxins and ergosterol in alfalfa hay.The methods of mycotoxins: Improved the methods of six kinds of mycotoxins. They are AFs, ZEN, DON, OTA, FBs, T-2, respectively. Experimented with precision, reproducibility, affix recovery test. In the experiment of precision, intra-day and inter-day precision, the relative standard deviation (RSD) was less than 3%. It showed that the instrument precision was good. In the experiment of reproducibility, the recovery was about 90% and the relative standard deviation (RSD) was less than 2%. As FBs needed chemical derivatization, the recovery was effected, but it was still high, about 80%. It showed that the experiment had better repeatability. In affix recovery test, the recovery was about 80% and the relative standard deviation (RSD) was less than 5%. According to above data, it explained that these methods were apply to measuring the six kinds of mycotoxins in alfalfa.The correlation between mycotoxins and ergosterol: Measured the six kinds of mycotoxins in alfalfa. There is only one mycotoxin, ZEN. It showed that the content of mycotoxins is ZEN content. However, the regression between ergosterol content and ZEN content showed that there was no significant correlation between them. It showed that there was no significant correlation between ergosterol content and mycotoxins content. According to above conclusion, it explained that we were unable to use ergosterol content to estimate mycotoxins content in alfalfa hay, reflecting the degree of mycotoxins infection.