| In this study, compound four kinds of single adsorbent between the different levels and added to the natural moldy broiler diets to screening the best combination and optimize validation.Using 4*3 orthogonal design, 432 one day old Ross 308 broilers were assigned to 9 treatments with 6 replicates of 6 birds each treatment. the experimental diets were designed to contain 0.1%, 0.2% and 0.3% montmorillonite;,0.02%, 0.03% and 0.05% cholestyramine; 0.02%, 0.03% and 0.05% glucomannan; 0.02%, 0.03% and 0.05% polyvinylpyrrolidone respectively. Chiekens were fed designed diets for 6 weeks of feeding trial, studied adding different montmorillonite, cholestyramine, polyvinylpyrrolidone and glucomannan adsorbent to moldy diet ( 50% mold corn replacement of the conventional diet) on the Ross 308 broilers (0 to 42d). Response variables measured were chick performance, organ weights, serum biochemistry, skeleton growth and immune function, selected an optimal adsorbent from combination.The results showed that: when the montmorillonite level of 0.3%, cholestyramine level of 0.05%, dextran level of 0.03% and PVPP level of 0.02%, in feeding stage the chicken can get the higher average daily gain feed intake; tibia calcium and phosphorus content was the highest; the highest serum total protein, albumin and concentration of lysozyme, the highest antibody titer to Newcastle; 21 days of age when the montmorillonite level of 0.2%, cholestyramine level of 0.05%, dextran level of 0.03% and the PVPP level of 0.05%, have the longest length, the maximum diameter and the maximum weight of tibia; 42 days, when the montmorillonite level of 0.3%, cholestyramine level of 0.05% glucan levels of 0.02% and PVPP level of 0.02%, have the longest length, the maximum diameter and the maximumweight of tibia. Comprehensive comparison of the index, in this experimental condition, the treatment group 9 sorbent combination: 0.3% montmorillonite, 0.05% cholestyramine, 0.03% glucomannan and 0.02% PVPP compound detoxification agent for the best combination.In experiment 2 144 one day old Ross 308 broiler chicks were randomly allotted into 4 groups, with 6 replications per group, 6 broilers in the replication. The 4 dietary treatments consisted of: (1) control, basal diet; (2) pollution group(moldy diet: 50% mycophenolate corn replacement group); (3) treatment group 1(pollution group diet plus commercial mildew agent); (4) treatment group 2 (pollution group diet plus moldy feed compound detoxification agent);The raising period was 6 weeks.Research in the diets naturally contaminated with mycotoxins diets and supplemented with commercial mildew agent and compound detoxification agent (from Experiment 1) effect on growth performance, immune function, calcium and phosphorus metabolism and biochemical indicators of blood, to verify the detoxification effect of compound detoxification agent. The results showed that: the two treatment group diets compared with the pollution diets could increase in different degree: broiler chickens daily feed intake, average daily gain and feed conversion ratio in various feeding stages of (P>0.05); bursa index,thymus index and spleen index (P>0.05); serum total protein, albumin, lysozyme, and Newcastle disease antibody levels (P>0.05), chicken tibial length, diameter and fresh weight (P>0.05), and significantly increased 21-day calcium level (p≤0.05). Also lower the serum cholesterol level (P>0.05), serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lactate dehydrogenase enzyme activity (P>0.05). The two treatment groups detoxification agent can alleviate the adverse effects of mycotoxins, compound detoxification agent is better than commercial mildew agent. |
