Yanbian Area The Bee Disease Epidemiology Investigation And PCR Detection Method Is Preliminary Established

"Yanbian Korean autonomous prefecture", hereinafter referred to as "yanbian area is located in the changbai mountains" Has a unique ecological environment and natural resources advantage, not only is rich in linden honey, but also with a wide variety of woody and herbaceous auxiliary powder plant, known as the "library" dongshan honey. Main quality nectariferous plant linden, lespedeza, thorns and so on more than 300. According to expert estimates, according to the existing nectar source area of the yanbian region can carry 800000 cases bee. At present,224000 bees group.Bees chalk disease (bee chalkbrood diseases) pathogens for bees balloon bacteria (Ascosphaera apis), is a kind of widespread bee larvae death disease, an important quarantine disease. Bees balloon fungus spores have strong resistance, can survive a year in pollen, can live 15 years under dry conditions. Disease have occurred in our country, and around the world in recent years in our country some areas of the swarm of bees infectious disease outbreak, as to the yanbian area turned to put a swarm of bees, bee disease transmission way and the opportunity to continue to increase, the acceleration of spread, bee disease has bee plague outbreak of the yanbian region top three, become the enemy of apiculture development, serious impacts on the development of yanbian region beekeeping and yield and quality of honey.Bees medium for the spread of disease is very broad, such as water, feed with bees are the main media of the transmission. Within group spread mainly through back office bee feeding activity, the pollen of brussel infect healthy larvae and disease, in addition, the mycelium can also infect directly larvae. Chalk after disease is difficult to control, with chalk swarm sick is wiped out whole group, while the group of potential is waning. Chalk disease epidemiological studies, therefore, appear very urgent and necessary.According to the bee disease epidemiology basic research, preliminary established a molecular diagnostic techniques based on PCR bees chalk disease, the molecular diagnosis technology has the characteristics of accurate, sensitive and fast, and not dependent on the typical symptoms, so can be used for buzzers no typical symptoms or the onset of symptoms is not easy to find the early or pathogen detection in the incubation period.This study provide theoretical basis for the bee disease epidemiological studies, to further screening functional genes, and lay a foundation for using PCR bees chalk of diagnosis and treatment of disease, and promote characteristic bee industry healthy and rapid development in China.