Studied On The Residues Of Quaternary Benzo[C]Phenanthridine Alkaloids And Primary Metabolites In Swine

In recent years, food safety problems of food-borne animal products has become the focus of social problems.How to solve excessive veterinary drug residues in livestock and poultry products, and ensure the food safety is an important subject faced by modern healthy breeding. In this context, traditional Chinese veterinary medicine is becoming a hot spot of new veterinary drug research. In order to promote animal growth, and ensure food safety from the source, Macleaya cordata extract has been developed into the first of second grade new traditional Chinese veterinary medicine, and as the first plant-derived drugs has been approved for use in feed additives to replace antibiotics in the livestock breeding, In this paper, the organs of swine which fed with fodder has added Sangrovit for90days used as the research object. Quantitative detection of the residues of sanguinarine, chelerythrine and the metabolites of dihydrosanguinarine, dihydrogenchelerythrine had been done.1. The swine muscle samples were prepared to experimental liquid by the process of ultrasonic extraction, centrifugal separation, enrichment and redissolve, and determined using the liquid chromatography-electrospray ionization mass spectrometry. The concentration of the sanguinarine and chelerythrine has a good linear relationship with peak area in0.2～20ng/mL concentration range. The average recovery was91.5%and91.3%respectively, and all of the methodological indicators had met the requirement for quantitative determination of two kind of alkaloids in swine organs. The method has high sensitivity, good repeatability, and suitable for the quantitative detection of the trace amounts of benzophenanthridine alkaloid in pork and organism.2. By optimizing the pre-treatment conditions, the optimum extraction and enrichment conditions suitable for dihydrogen sanguinarine and dihydrogen chelerythrine was established. And a specificity, high sensitivity and good reproducibility detection methods was established by optimizing the various conditions of liquid chromatography, All of indicators of this method were validated by methodology, and suitable for quantitative detection of trace amounts of dihydrogen sanguinarine and dihydrogen chelerythrine in swine tissues.3. By the experiment of feeding swine with Sangrovit for90days and setting different withdrawal periods, the drug residues of four alkaloids in swine tissues were quantitative determined using the established methods of detection of the four kind of alkaloid. According with the results of the remaining amount of four kind of alkaloids in the tested tissues, and with results of the acute and chronic toxicity and the safety evaluation tests of Macleaya cordata extrac, we can concluded that the carnivorous products which fed with sangrovit are safe.