A LC-MS/MS Method For The Detection Of Major Veterinary Drug Residues In Foods

While the requirements of quality and food safety are changing stricter, the harmful accidents of food safety are frequetly reported, the cases of some immoral producers and sellers of foods using forbidden drugs, overdoing drugs, not observing related legal laws often happen. All these ring alarms for food safety admistrators and propose new requirements for related researchers. How to effectively, quickly, and sensitively determine the trace contents of animal drugs becomes an urgent task. Liquid chromatography tadem mass spectropy (LC-MS/MS) is one of the main food safety analyitcal technologies, which show special advantages to seperation and identification of high boiling point, involatile and Thermo-instable compounds, and high seletivity and sensitivty to trance analysis. The main aim of this research is to follow and target on the morden international residue analysis technolgies. By systemic studying including extraction, cleaningup, determination and positive confirmation etc., using LC-MS/MS, a serial testing methods of determining animal drugs in foods and the followed standardization have been developed, as acts important effect, builds stable base, solve many technical questions for food safety and related areas in China. The research is divided into three parts. The first part is the studying of testing technolgy of nitroimidazoles in animal original foods, the second part is the studying of testing technolgy of fluoroquinolones in animal original foods, the third part is the studying of testing technolgy of sulfonamides in animal original foods. The tested compounds in the yielded methods to determine three animal drugs in animal original foods, cover and satisfy the current domestic and international requirements, the limits of detection conform to the current domestic and international MRL requirements. The testing periods compared to the current mothods are shorten 50%, and the costs are shorten 60%, as makes the residue analysis of China step the same pace as the internation level.