A Preliminary Study On The Impact Of Microelement Selenium On Endometritis In Dairy Cows

Selenium (Se) is an essential nutritional trace element. Se acts as modulator ofinflammatory response in infectious and autoimmune disease and plays a pivotal rolein enhancing the body immunity and resistance. Dietary Se concentration is associatedwith many inflammatory diseases, especially reproductive obstacle disease.Endometritis is a reproductive obstacle disease and is characterized by mucous orpurulent inflammation occurring in the uterine tissues. Therefore, the major objectiveof this study is to investigate the effects of dietary Se on endometritis.In order to investigate the effects of dietary Se on endometritis, we investigatethe management of the production and the incidence rate of postpartum cowsendometritis, detect the content of Se in cows postpartum uterine tissue on the cattlefarms in Qingzhou of Shandong and Fengman District of Jilin Province. The resultsshowed that, the situation of production management was different, so the incidenceof postpartum endometritis cows was different. The incidence rate of cowendometritis was22.2%in poor production management of Jilin fullness area, wasmuch higher than16.3%in Qingzhou of Shandong. The determination result of Secontent in uterine tissues showed that the average concentration of Se in cow withendometritis was lower than that in cow without endometritis in two cattle farms. Theincidence of cow endometritis was negative correlation to the Se content in uterinetissues in the cattle camp. These studies demonstrated that the incidence of cowendometritis is connected with Se in the cattle farm.Endometritis often occurs in postpartum caused by a variety of pathogens.Escherichia coli, one of the main pathogens, often causes clinical endometritis.Lipopolysaccharide (LPS), which is the main component of the outer membranes of E.coli, can trigger the consistent inflammation response of E. coli. Since we have foundthat microelement Se may be associated with endometritis in dairy cow. The animalswere fed with dietary that contain the different Se level, and an intrauterine infusionof LPS was used to generate a model of mice endometritis. The Se content, histopathological analysis and pro-inflammatory genes analysis were conducted tostudy the effects of dietary Se levels on LPS-induced mice endometritis. Resultsshowed that the role of Se in uterine tissues was affected by dietary Se levels in a dosedepended way. Histopathological observation of uterine tissues in LPS-inducedSe-deficient mice endometritis showed that many of the epithelial cells weredestroyed and that the uterine tissues were infiltrated with a host of inflammatory cells.Pro-inflammatory genes expression levels in uterine tissues of Se-deficient groupwere higher than Se-adequate group. These studies demonstrated that Se deficiencymakes mice uterus more prone to inflammation. An appropriate Se supplement couldalleviate inflammation of the uterus. These results may provide reference to reducethe incidence of cow endometritis for the veterinarians.