Development And Evaluation Of A Mixed Microbial Inoculant For Speed-up Of Chicken Manure Composting

With the high speed development of livestock and poultry industry, the amount of animal manure dramatically increased in China. However, the traditional manure disposal is low efficient and time consuming. To solve these problems, the mixed microbial inoculant was developed to speed up the compost process in the present study.To evaluate the impacts of the bacteria on compost maturity of chicken manure, dominant bacteria screened from chicken manure and commercial bacteria were used to develop the mixed microbial inoculant. The main findings in this study are listed as follows:(1) With the selective medium, cellulose degrading bacteria including5viable bacteria under27℃,7viable bacteria under37℃and4viable bacteria under50℃were isolated as low or medium temperature active bacteria from manure compost and9bacteria under60℃were isolate as high temperature active bacteria. Mixed with7functional strains from the China Agricultural Culture Collection Center, the ABTNL#1inoculant was produced and inoculated into the manure compost built in September2013.(2) The effects of mixed microbial inoculant on temperature during chicken manure composting process were evaluated in the present study. The results showed that the time needed for temperature reach to50"C in experiment group with the ABTNL#1inoculant was2days shorter compared with control group, and the duration of50℃above stage was8days longer in experiment group. Furthermore, the highest temperature in compost with the ABTNL#1inoculant was70℃, compared with57.5℃in control group. The results indicated that the effectiveness of ABTNL#1inoculant in speed-up of composting process of chicken manure. The high temperature generated in compost is responsible for the inactivation of pathogens in chicken manure.(3) The effects of ABTNL#1on compost maturity were investigated in further study. The results showed that experiment group with additional ABTNL#1inoculant generated an earthy and humic odor without ammonia or fecal odor after60days composting, while the control group showed a hint of ammonia and unpleasant odor after composting. Compared with an45%seed germination index (GI) for compost effluent in control compost sample, the mixed microbial inoculant increased GI to88%after30days composting. In addition, the microbial inoculant increased GI to90%after60days composting, while GI in control group was only66%. In conclusion, the ABTNL#1inoculant accelerated the decomposition of toxic and hazardous substances in compost, and promoted the process of compost maturity. After composting, the manure compost product could be used as an organic fertilizer, which developed a new way for resourcing process of manure waste in livestock and poultry industry.