Studies On Agaricus Bisporus Residue Compost, Changes Of Bacterial Population And Its Fertilizer Efficiency

In this study, we taking agaricus bisporus residues as investigated subject, using molecular biology method and traditional compost method to investigate the change of bacterial communities and fertilizer efficiency in the compost of the microbial inoculant or chicken manures. The main results of this study as follows:(1) The changes of temperature, pH value, EC value,during the composting of agaricus bisporus residues. By adding microbial inoculant or chicken manures into the Agaricus bisporus residue compost, can raise the temperature of the compost quickly and shorten the composting time; degrade the organic acids produced effectively during composting and other acidic substances; to ease the decline of pH in compost. The changes of CE value in the compost showed some fluctuation, but overall changed slightly. By composting can effectively reduce the harmful ions damage to crops,which in Agaricus bisporus residue.(2) Studies on Agaricus bisporus residue compost and its fertilizer efficiency. In this research taking Agaricus bisporus residues as investigated subject. By adding microbial inoculant or chicken manures into the Agaricus bisporus residue compost, the change trends of total nitrogen, total phosphorus and total potassium at different periods in the compost preparation and in different treatments are analyzed. The performance of the residue compost on paddy rice are studied as well. The result shows that the addition of microbial inoculant during the preparation of Agaricus bisporus residues compost could rapidly increase the compost temperature. Compared with the treatment A, the contents of total nitrogen, total phosphorus and available potassium in the compost added with the microbial inoculant are increased by 3,1.43 and 2.67 times, respectively. After composting, the content of available phosphorus in the treatment A, treatment B and treatment C increased by 54.5%,38.5% and 58.3% respectively. The field experiment of the residue compost on paddy rice indicates that organic fertilizer of Agaricus bisporus residues could promote yield increase of paddy rice. The residue compost has better effect on crop yield than that of non-fermented residue. The residue compost added with microbial inoculant has the best effect. With fertilizer application at 400 kg/mu, the paddy rice has less empty grain per ear and the yield reaches at 553.37 kg/mu, while the yield increased by 20.55% compared with that under conventional fertilizer application treatment and by 44.18% compared with that with no fertilizer application treatment.(3) Changes of bacterial communities in Agaricus bisporus residue compost. PCR-DGGE (Polymerase chain reaction-denaturing gradientgel electrophoresis) analysis was used to study the change of bacterial communities during composting of Agaricus bisporus residues. Composts were sampled during different composting periods, and the changes of temperature were measured. The results showed that the electrophoretic bands of treatment C was 24 at high temperature period of compost,which was significantly higher than treatment A(the electrophoretic bands was 14 at seventh day), By adding microbial inoculant the content of thermohilic bacteria was significantly increased and population structure of bacteria in compost was changed. The Cs value of two adjacent stages in treatment C were 76,71,73,662,Adjacent stages of composting bacteria population that little difference, the change is relatively stable. By Quantity One software, the number of DGGE bands of spectrum and brightness of digital processing, and so as far as cluster analysis, DGGE profiles can be divided into two categories, the treatment C was a class, the treatments A and B were the other class. The result shows that the addition of microbial inoculant during the preparation of Agaricus bisporus residues compost has a significant effect on the bacterial population.