Tissue Culture Of Siraitia Grosvenorii Space Seed And Characteristic Study Of Tissue Culture Seedlings Growth In The Field

Siraitia grosvenorii, a member of the gourd family,belongs to perennial grass vines, and it is recorded in the pharmacopoeia of People’s Republic of China as a unique medical and edible plants, Siraitia grosvenorii is a kind of potential resource of natural products. Siraitia grosvenorii is cool in nature and sweet in taste, and the fruit is often used as medicinal materials. It has efficacy of cleaning lung-heat, stopping cough, dissolving sputum, relaxing bowel, protecting liver and anticancer properties so that it is widely used in medicine, drinks and flavouring agent. It is mainly distributed in GuiLin, Guang Xi province and the damp mountain area in Gui Zhou province and Yun Nan province.The technique of space seed breeding, as a kind of new breeding technique, is an effective new ways to breed new excellent variety fast. In November 2011, Siraitia grosvenorii launched smoothly with the Shenzhou eight spacecraft, through the influence of the complex space environment, seed changes in mass and characters, which may bring new breakthrough to the area of seed breeding of Siraitia grosvenorii. But space seed breeding is expensive,Therefore, it is important to bring the Siraitia grosvenorii seeds with higher value. Use of aerospace breeding and production technology to improve the output Siraitia grosvenorii is of great significance. In this paper,physiological features, proportion of male and female and chemical composition of the fruits between the space seedlings and the normal seedlings were studied. This results will take a certain application value for choosing good quality and female siraitia grosvenorii. The main research results are as follows:1. Firstly space seeds of Siraitia grosvenorii were divided into the upper, middle and under, and then these three different sections of the seeds were put into the same medium for germination, transgeneration, taking root in the laboratory. Finally these seedlings were plant in the field. After a period of time, the length, width and the diameter of the eighth leaf of all plants were measured. Under the condition of single factor measurement, the results showed that among these three parts, the leaves of middle seed plants were obvious better than that of the other two in the aspect of the length, width and the diameter. The leaves of the control group plant were longer and wider than that of space seed distinctly, and the stem diameter of the plant of the experimental group was also relatively thinner than that of the plant of the control group.2. The chlorophyll in Siraitia grosvenorii leaves was separated and extracted. According to the absorption of visible light spectrum, the absorbance was measured under the particular wavelength with the spectrophotometer, and the content of the pigment in the extract was calculated. The results showed that among all of plants from the upper middle under seeds, the leaves of middle seeds contained the most thlorophyll. The thlorophyll in the leaves of the control group seeds was more than that of the space seeds.3. The quantitative measurement of protein content in different leaves was determined with coomassive brilliant blue method. The results showed that among all of plants from the upper, middle and under seeds, the leaves of middle seeds contained the most protein, the protein content in the leaves of the upper seeds was more than that of under plants. The average protein content in the leaves of the control group seeds was higher than that in the experimental group seeds.4. The soluble sugar content in the leaves was measured with Anthrone colorimetry. The result showed that the soluble sugar content in the leaves of normal seeds was more than that of the space seeds distinctly. Among the plants of same seeds, the soluble sugar content in the leaves of middle seeds was a little higher than that of the leaves of upper and under plants, but no marked difference.5. Siraitia grosvenorii leaf relative electrical conductivity was measured by soaking method, its measurement results showed that in the same seeds of Siraitia grosvenorii fruit, the relative electrical conductivity of the leaves from the middle section seeds was the highest. But compared with the experimental group, the relative electrical conductivity of control group relatively was higher.6. Using guaiacol method determination of the activity of peroxidase in Siraitia grosvenorii leaves, the results showed that the activity of peroxidase (pod) in the plant of middle seeds was highest, and that of under seeds plant was lower than middle seeds plant. the activity of peroxidase (pod) was the lowest in the plant of upper seeds. The activity of peroxidase (pod) in the experimental seed formation of plant leaves was higher than that in the leaf of the control group.7. The proportion of male and female plants are different in the seeds of different section in the same Siraitia grosvenorii fruit. Male strains was more than female strains in upper seeds. No obvious difference was found between male and female proportion in Middle seeds. On the whole, the proportion of male strains was higher. The experiment showed that the same results were obtained in the control group.8. Using HPLC method to determine sweet glycosides Ⅵ content in the experimental group seedlings and the control group seedlings fruit, the results showed that the sweet glycosides V content in experimental group was significantly lower than that in control group.9. Using HPLC method to determine the experimental seedlings and the control seedlings of fruit zhongshan nai of phenol and the content of quercetin, and then the content of total flavonoids in these two kinds of fruit was calculated. Measurement results showed that the content of total flavonoids of phenol content in the experimental seedlings of fruit was significantly lower than that in the control group, Come to the conclusion by calculation, the content of total flavonoids of the middle seedlings fruit was higher than that of upper and under seedlings fruit.10. The determination of using ultraviolet spectro photometry shows that vitamin C content of the upper seed fruit was the highest. But compared with the experimental group, vitamin C content of the control group was higher.