Screening Of Salt Tolerant Clones In Potato

Potato with wide distribution, strong adaptability, high yield, rich in nutrition, and other characteristics, with appropriate grain, appropriate food, appropriate feed, industrial raw materials for a variety of purposes, such as staple food grain and economic crops should do. In recent years,the soil salinization on potato growth and development caused great threat,The development of salt tolerant materials of potato had become a new research field.This experiment was based on five potato varieties as the test materials,Selection of microtuber as explant for callus and obtaining salt tolerant clones.Salt tolerant clones grams Kenxin 4-1-200, Kenxin 18-1-200, Kenxin 18-3-150 and Kexin 23-3-150 subculture multiplication. After cultured medium were added Na Cl salt stress treatment, and from two aspects of the morphological and physiological characteristics of for salt tolerance evaluation. The main results are as follows:(1) To plant height, plant fresh weight, root fresh weight and stem diameter of the four morphological index comprehensive evaluation of potato de virus free plantlets resistant to salt,the appearance, amplitude variation and significant analysis,The stem diameter was increased on the salt stress,the plant height,and the shoot fresh weight and the root fresh weight were decreased.draws the salt stress on potato virus free plantlets of stem diameter influence is the largest,followed by plant height, resistance of low salt plant fresh weight and root fresh weight changes were more obvious.(2) After the determination of physiological index: after salt stress treatment, and the chlorophyll content of virus free plantlets are declining, and salt sensitive varieties decreased to a greater extent than salt tolerant varieties; root vigor decreased, and salt sensitive varieties decreased in salt tolerant cultivar; proline content the upward trend, and the changes in salt sensitive varieties than salt tolerant varieties; the trend of SOD and POD two kinds of protective enzyme activities are not consistent, different genes materials showed different trends in salt stress,the material itself and related enzymes play role, the ability to sum up, the effective index of chlorophyll content and root activity can be used as the evaluation of salt tolerance, and the proline content and protective enzyme activity can be used as a reference index for evaluation.(3) The experiment takes potato mini potato as explant, and distinguishes it from the leaves and stems of the former leaves.. Per liter of culture medium added 1ml ZT and 4ml IAA callus tissue culture, induction rate reached 85%, the proportion and concentration of this hormone were suitable for callus induction of salt tolerance.(4) The salt tolerance of the test tube seedlings of the five potato varieties was from strong to weak order:Kexin18>kenxin4>Kexin13>Kexin25>Kexin23,salt tolerance of salt resistant clones is from strong to weak order:Kexin 18-3-200>Kexin 4-1-200>Kexin 18-1-150>Kexin 23-3-150.