Detection And Analyses Of Tuberculosis And Brucellosis In Dairy Cows In Jingbian

Dairy cow industry plays an important role in agricultural structure and economic development around the world. Recently, dairy cow industry has been advancing rapidly in China. The quality and safety of animal products are closely related human health, and beef, milk and fur products are necessities for humans in modern society. Tuberculosis and brucellosis in dairy cows have great zoonotic potential. The intensive farming of dairy cowsin Jingbian is becoming a new growth point for rural economy. Based on the current situation, the objectives of study were to detect the two infectious diseases in dairy cattle from 2010 to 2014 according to recommend methods in order to supply references for controlling local tuberculosis and brucellosis.1. From 2010 to 2014, a total 11515 of dairy cows were detected with the national testing standards(GB/T18645-2002)-tuberculin intradermal allergic reaction for detecting tuberculosis. The average positive rate of tuberculosis in dairy cowswas 0.72%(83/11515). In 2010, 368 of dairy cows were involved, and no positive cow was detected. The positive rates in 2011, 2012, 2013 and 2014 were 2.69%, 0.12%, 0.50% and 0.18%, respectively.2. From 2010 to 2014, a total 11515 serum samples of dairy cows were detected withBengal plate agglutination test, the average positive rate was 1.19%(137/11515).The 62 serum samples were positive, and the positive rate reached the peak at 2.69% in 2011, while 7 serum samples were positive, the positive rate minimized at 0.20% in 2013. To verify the accuracy and reliability of Bengal plate agglutination test, all positive samples were employed with tube agglutination test. The test results of two methods(Bengal plate agglutination test and tube agglutination test)were the same, showing coincidence rate at 100%.