Isolation Of Microbes And Optimization Of Its Fermention Conditions On Fish Scraps To Produce Microbial Fertilizer

Nowadays, a large part of by-product of fish processing are as rubbish and pollute the environment.This experiment screened bacteria that can ferment by-product of fish processing into useful nutrients for plant.The fertizer has the ability of phosphate-solubilizing and potassium-solubilizing and it has enough nitrogen for plant.This test selected 54 bacteria from microbial fertilizer, soil and rotted fish, and did the single fermentative experiment for fish scraps, the nitrogen fixation, phosphate-solubilizing and potassium-solubilizing experiment, antagonism synergy test, experiments of morphologic observation, physiological and biochemical test, molecular biology experiment to screen 5 bacteria(Acidovorax temperans, Pseudommonas stutzeri, Paenibacillus sp.,Aspergillus niger,Bacillus subtilis).The conditions of inoculum enlargement for compound bacteria: the medium( peptone 15 g/l, glucose 20 g/l, Mg SO40.03 g/l,Na Cl0.01 g/l,K2HPO40.15 g/l),temperature 30℃,p H=6.5, inoculation quantity 5%, speed 140 r/min,time 4-6h.The conditions of fermentation: fish:water=1:7, temperature30 ℃, p H=5.5, inoculation quantity 10 %,speed 170 r/min.Scale-up test of fermentation under the condition of fish:water=1:7,temperature 30 ℃,p H=5.5.Using fermenter of 7.5 L,50 L,1 t to research the speed, inoculation quantity and supplemental resource, in the last, the three fermenter got the same result(speed 190r/min, inoculation quantity15 %, glucose 30 g/L), and organic matter of the fertilizer was up to the standard,and it has the ability of Phosphate-solubilizing, potassium-releasing and nitrogen-fixing.This research can solve the problem:the pollution of fish waste to environment,long time of the production for farmyard manure, pollution of chemical fertilizer and transforming the waste into wealth.