Isolation And Identification Of Swine Intestinal Microbes With Deoxynvialenol Biodegradation

Deoxynivalenol (DON ) ( vomitoxin, VT ), is mainly composed of a secondary metabolite produced by some Fusarium - a trichothecene toxin, is currently the world's most polluted mycotoxins of corn, to humans and animals caused significant harm. In all the susceptible animals, pigs are the most sensitive animals, loss of appetite or waste vast, vomiting, weight loss, abortion, stillbirth and weak young. DON toxigenic matrix is mainly wheat and corn grain, this toxin pollution situation is very common, feedstuff and feed raw materials in the detection rate and the detection amount is quite high. Therefore, it is necessary to study how to degrade DON in feed on pig causing anorexia and vomiting toxicity, for the prevention and control of porcine DON poisoning and provide a theoretical basis and guidance for practical production. Currently, anti-mildew agent mostly via adsorb mycotoxin to remove mycotoxin on the market, but some nutritional factors had adsorbed by the type of preparation, and most of the mycotoxin adsorbent have no adsorption to DON. Two strains isolated anaerobic bacteria R1, R4 and one plant bacillus YB4 were separtated by the gastrointestinal tract in pigs using the MRS and nutrition agar medium in this experiment. Three stains were cultured after 24 hours ,then training with 1 mg/ml DON standard solution to DON final concentration of 1 ug/ml. Liquid was sampled respectively in 27h, 30h, 33h, 36h, 39h for LC-MS detection. LC-MS detection results showed that, bacillus YB4 had no degradation ability of DON, R1, R4 had certain degradation effect, the degradation rate were 16.7% and 23.2%.For the study of the R1, R4 on DON degradation mechanism, two strains of anaerobic bacteria and 1mg/ml DON standard solution while adding in 100ml of MRS liquid medium, liquid was sampled respectively in 6h, 12h, 18h, 24h, 30h, 36h, 42h for LC-MS detection. LC-MS detection results showed that there were necessarily positive relationship on the total bacteria number of two anaerobic bacteria R1, R4 and the degradation efficiency of DON. Colony morphology, gram stain and API50 CHL identification system results showed that R1, R4 were Lactobacillus plantarum and Lactobacillus acidophilus. In vitro experiment showed that the R1 and R4 strains could significantly degrade DON, but YB4 strains had no significant effect. Therefore, this research aims to develop a variety of microorganisms and enzyme based biodegradable agents, laid a theoretical basis for the prevention and control of porcine mycotoxin poisoning in pig production.