Studies On The Low-cost In The Rapid Propagation By Tissue Culture Of Floribunda Roses

The Floribunda rose ‘pink-A’ is cultivated by the research group of garden plant and ornamental horticulture of Northeast Agricultural University, which is popular because of the colorful, fragrant, concurrent flowers with a wide range of application. They are irreplaceable materials for landscaping in modern cities. Due to climate reasons in the north of China, there are few cultivars of roses can live through the winter, needing a large number of seedlings to promote the application of the link. Tissue culture and propagation technology can be resolved in the cutting propagation of the cuttings, such as the slow propagation of the spike, and the slow propagation of the propagation. It can propagate a large number of seedlings in a short time. So it is the first choice for the application of the tissue culture to promote the rapid propagation of the Chinese rose flower as soon as possible. However, the existing problems of the higher cost of the tissue culture of the mature Foribunda rose, only reduce the propagation of the cost of inputs, make greater economic benefits in practical application, social benefits and ecological benefits.Establishment of direct differentiation propagation system of Floribunda roses‘pink-A’,A set of tissue culture plan for the growth of plantlets was selected, and the primary culture vessels of the type and of medium preparation process and preparation of raw materials used for selective preparation of raw materials used in experimental study on selective test research. Comprehensive analysis and searching for the production patterns of the low cost organization of the high cost of the flowers and roses. To provide theoretical basis for the cultivation of the scale and the factory of the scale of the rose flowers, the main results of this study are as follows:1.Direct differentiation propagation system of Floribunda Roses: The medium is MS,distilled water,sucrose7.8g/L,Agar7.8g/L. The best medium for adventitious buds:MS+6-BA2.0mg/L+NAA 0.05 mg/L,the induction rate reached 94.61%. The best medium for adventitious buds the rate of proliferation can reach 4.32. The best rooting medium:1/2MS+NAA0.05-0.1mg/L, rooting rate can reach 94.17%.The most suitable transplanting substrate for a single matrix: peat, the survival rate of open field planting can reach 95.33%.2.In large-scale and factory produced tissue culture propagation of Floribunda Roses, some typies are as follows to reduce the production cost in the culturing of Floribunda Roses tissue:(1)Used with cover breathable plastic tube instead of 50 ml glass flask as primary culture container, reduce 73.33% of the container, cost saving 71.43% of display space, can prolong the effective time of the medium.(2) In the each stage of the tissue culture experiment, the medium of the water was used instead of distilled water, and the water quality cost was decreased by 73.60%.(3) The agar for low cost carrageenan as gelling agent, concentration by 7.8 g / L drop 6.5 g / L, gel results on seedling growth had no effect, can reduce 68.75% gel agent cost.(4)Provide to the culture medium carbon source for 30 g / L sucrose replaced with 25 g / L of white granulated sugar, not only to test showed no effects and can reduce the cost of 77.08% of carbon source.(5)In the process of preparing the medium, removing the step of heating the culture medium, directly to the high temperature and high pressure sterilization, the culture medium has no effect on the growth of the rose tissue culture seedlings.(6)Taking the subculture 3 times as an example, the cultivation cost of the plant tissue culture seedlings of the rose ’pink-A’ per plant can be reduced from 0.054 yuan to 0.012 yuan, and the plant tissue culture seedling is reduced by 77.78%.And the cost margin of the reduction is positively related to the increase of the number of generations.