Common Wintercreeper Euonymus Highly Effective Low Cost Tissue Culture Breeding Technology Research

Euonymus fortunei in the arid zone greening cities and towns and desert in the governance of great prospects for the development of the climbing plant.Euonymus fortunei fast growth of branches on the ground,man climbing ability,well-developed root system; cold,barren resistance in different site conditions,with strong adaptability.The purpose of this study is to choose the appropriate medium,tissue culture to reduce the rate of pollution, to overcome Euonymus fortunei explant browning and glass,using tissue culture alternatives to reduce costs and simplify work processes,further in-depth research,help to establish Teng Fang high-performance low-cost technology of rapid propagation system,bring the advantages of tissue culture,tissue culture so that a large number of rapid propagation of Euonymus fortunei become the main way in a short period of time to obtain a large number of specifications,robust growth,good growth of Euonymus fortunei Seedlings,specifications, robust growth,Euonymus fortunei good seedling growth,the rapid application of this excellent plant shrubs to make it in urban greening the desert and play an important role in governance.The study of in vitro rapid propagation of Euonymus fortunei positive guidance.The results show:①The most appropriate medium for the growth of Euonymus fortunei MS;2,4-D in the callus induction plays an important role,6-BA on the induction of buds have a major impact on the role.When 2,4-D concentration of 0.05 mg/L,the callus induction rate to increase dramatically;6 BA is a high concentration of also increased the number of callus,When 6-BA concentration of 1.0 mg/L,produced the most callus;when 2,4-D concentration of 0.1 mg/L,the callus induction rate has declined.When 2,4-D concentration of 0:00,the bud-induced high rate of 6 as a high concentration of BA,bud induction rate as the rise.When 6-BA concentration of 1.4 mg/L,the highest bud induction.When 2,4-D and 6-BA-exist,at the same time facilitate Calli and the induction of bud,bud,but the rate of induced only an additional 6 as a high BA.②NAA Euonymus fortunei on the proliferation of renewable Miller IAA than good.When additional NAA concentration for only 0.1 mg/L, robust plant growth,proliferation soon;With the increased concentration of NAA,plant growth is getting worse,the proliferation of slow growth;Proliferation of seedlings Euonymus fortunei renewable portfolio train the best medium for MS +6-BA1.0mg/L+NAA 0.1 mg/L.Miller renewable robust growth,rapid proliferation of plants,the proliferation reached a multiple of 3.25.③NAA,IAA root effect is better than IBA.In vitro induced by the time of the transplant survival rate significantly affected,with the time of the light-induced increase in the rate of in vitro pollution has increased,but by a high degree of Miller,the survival rate of increase.Euonymus fortunei in vitro root Baptist 10s and then transplanted to promote root agent IAA60mg/L+IBA30mg/L to deal with the situation of the children survived the best plants,in vitro perlite,peat,peat and slag 1:1 mixture of 3 Matrix in 30 days after the transplant,the survival rate for the best perlite,the survival rate of 85%.④Select the appropriate explant culture and to establish the best conditions to overcome the material is the primary means of browning.Materials in the pre-vaccinated after a certain pre-ease in the explant culture of browning.In the process of training, low-temperature heat than is conducive to inhibit browning.Vc strong inhibition of browning, but also inhibit the growth of explant differentiation;inhibit browning effect of activated carbon,but inhibit differentiation.Different way of training caused by the degree of browning, Calli and the media to increase exposure of the oxygen supply is conducive to inhibit browning,the replacement of fresh medium,the rate of browning can be significantly reduced.⑤Appropriately increasing the light intensity can help to overcome the glass; appropriate medium to lower concentrations of NH4 +,pay attention to the types and concentrations of carbon source,pay attention to cope with the growth hormone cytokinin and,taking into account the proliferation of adventitious buds coefficient of glass and control the occurrence of Miller.Concentration of sugar 3g/L in vitro Euonymus fortunei reduce the occurrence of glass;0.3g/L of activated carbon medium in vitro rapid growth,a lower rate of glass.High temperature will be detrimental to the growth of material culture,and even in vitro will lead to the glass aggravating phenomenon.When the temperature 25℃,while the glass is not the smallest rate of seedlings,but the normal number Miao Miao Development of robust,relatively fast growth rate.⑥Pre-transplant training is very important to strengthen the transplanted tissue on the management of rapid propagation of Euonymus fortunei is to increase the production of seedlings into the main factor measures.Humidity and light affect the survival rate of transplanted tissue culture of the main limiting factor,keeping a matrix of water,light intensity can not be transplanted too early for the benefit of stem tissue culture of root development,improve the survival rate.