Genetic Diversity Of New Wheat Cultivars In Southern Huang-huai Area In2013

In orger to understand huanghuai area wheat breeding new varieties of wheat latestagronomic traits and the composition of HMW-GS and genetic diversity, this study of2013in huanghuai wheat south of winter and spring wheat pilot study of143wheat varieties,through field investigation and analysis of main agronomic shape difference. The wheatgenetic stocks were analyzed by field agronomic, HMW-GS compositions and SSR markertechnology. The principal results are as follows：1、Analysis Was conducted on the basis of6investigated quantitative characters between143wheat genetic stocks．The results showed that the test material had few spikes per plant,there is a big difference in the shape little more than kernel number of70, mainly between40and60. There is a little differences among the varieties, and is less than80cm tall. Ear lengthmainly concentrated in8-11cm; Thousand seed weight was greater than40g, and only4.19%of the material is less than40g. The scope of the spikelet number is18-26distributed evenly.We can see that huanghuai wheat varieties of wheat plant individual number of spike length,spike grain number and have a certain genetic differences, and the spikes and seeds per ear onthe two shapes have larger room to improve.2、In order to understand the component situation of high molecular weight gluteninsubunit(HMW-GS) and the quality of new wheat varieties in Huang-huai wheat region,HMW-GS compositions of143materials were analyzed by SDS-PAGE. The results showedthat there were detected10HMW-GS alleles and15subunits compositions, Subunits1atGlu-A1was found with frequencies of59.44%,Subunits “7+9”at Glu-B1and Subunits“2+12”at Glu-D1.The major subunits compositions were “17+92+12” and “N7+92+12”,high quality subunits compositions were “17+85+10”and”114+155+10”, besides, therewere16varieties with quality score10and the averaged quality score was7.32, whichindicated that the quality was improved and the quality subunits “7+8””17+18” showedhigher frequencies than before. However,2*at Glu-A1was not detected so rare qualitysubunits should be taken into consideration in the future wheat breeding.3．The genetic diversity analysis of wheat samples showed that polymorphic variencewas observed in the21pairs of SSR primers，81equipotential variance was detected, and the average is3.9; and the average of polymorphic information coefficient is0.29.So it can beseen that huanghuai wheat has less genetic diversity degree any more. From the perspective ofthe average number of allelic variation of materials needed for wheat, genome D> B> A. PICorder to an average of three genome is respectively: B genome> D genome A genome. Bgenome genetic diversity is rich, D genome is second and A genome is low, and A genomeallelic variation in the uneven distribution of varieties. Through clustering analysis can bedivided into four categories, the selected material of which127bound for a class of materials,accounting for88.81%of the total,16other varieties were was divided into three categories.This research shows that some varieties exist good genetic diversity, but the genetic diversityof huanghuai wheat new strains of richness was poor.