Analysis Of Genetic Dversity On Genus Paulownia.et Zucc Plants

Paulownia (PaulowniaSieb.et Zucc) for the ScrophulariaceaePaulownia is a deciduous tree, its natural distribution or planting areas is all overthe country23provinces (city)and the autonomous region.it is an important type ofthe good quality and fast-growing timber species. Paulownia is cross-pollination,hybrid, and can through asexual reproduction, and easy to form, retain the transitionaltype. The traditional method of morphological analysis is to study the geneticvariation of plants using phenotypic characters, effects are often subject toenvironmental factors such as external conditions, can not truly reflect the geneticvariation. In order to clarify Paulownia and variants of genetic variation and geneticrelationship, genetic diversity analysis method was used in this study AFLP and ISSRtwo kinds of molecular marker combination of Paulownia plant sex and relationship,understanding and Geographical Provenances of Paulownia Plants mastery of geneticvariation and internal reasons in order to better, and provide a theoretical basis andreference data for the reasonable use of plants of the genus Paulownia and geneticbreeding, providing important theoretical guidance for seed breeding, suitable trees,creating high productivity of artificial forest. The main results are as follows:(1) two kinds of methods with CTAB method, SLS method and modified fromPaulownia genome DNA, and detect the quality and concentration of DNA, comparedto the two methods, results show that the extracted by improved CTAB method ofDNA of higher quality, more suitable for AFLP for ISSR amplification demand.(2) based on AFLP molecular marker of Paulownia Plants of14species andvarieties, genetic diversity analysis, results showed: select9pairs of PstI/MseIcombinations from64pairs of AFLP primers, and1171bands were clear, of which1158bands are polymorphic, percentage of polymorphic bands reached98.89%,14accessions of AFLP genetic similarity coefficient ranged0.603~0.740, with anaverage of0.672. And the threshold of0.675,14accessions were divided into4groups, namely P. fortunei, P. australis, P. kawakamii, P. fargesii, P. ichangensis, P.jianshiensis, P. elongata f.alba, P. albiphloea and P. elongata in the first group; P. tomentosa, P. lamprophylla and P. tomentosa var.lucida in the second groups;the thirdgroup and thefourth group contains a varietie,respectively P. catalpifolia and P.albiphloeavar.chengtuensis.(3) in this study, Paulownia Plants14species and varieties as experimentalmaterials of ISSR molecular markers, the experimental results show that: from theISSR published primers were screened in11clear, good repeatability, highpolymorphism, for Paulownia14accessions were amplified by ISSR-PCRamplification,90clear bands, including82polymorphic bands, the average of eachprimer amplified polymorphic bands was7.45, the percentage of polymorphismbetween60%~100%, the average polymorphism detection rate was91.1%. Clusteringresults showed:14accessions were clustered into3groups: the first group include: P.tomentosa, P. lamprophylla and P. tomentosa var.lucida; The second group include: P.catalpifolia,P. ichangensis, P. albiphloea, P. elongate, P. fargesii, P. jianshiensis and P. elongataf.alba;The third groups include: P. fortunei,P. australis, P. kawakamii and P.albiphloeavar.chengtuensis.(4) The experimental results shows that：AFLP markers and ISSR markers of Paulowniagermplasm materials significantly correlated to the results of analysis of genetic diversity,correlation coefficient r=0.386(n=91, P=0.01), showed that these two kinds of molecularmarkers used for paulownia germplasm genetic diversity analysis of the material has highconsistency.