Inhibitory Effect Of Traditional Chinese Medicine On Mycoplasma Suis In Vitro

With the rapid development of animal husbandry in China, mycoplasma suis disease incidence continues to increase;With the pig industry has brought economic losses.Mycoplasma suis disease prevention mainly for medicine, a wide variety of drugs, the effect of uneven, resulting in indiscriminate use and abuse of drugs, This phenomenon not only led to a resistant mycoplasma suis disease, but also cause drug residues and impact on food safety. Therefore urgent need of inhibit screened mycoplasmasuis of traditional Chinese medicine, for mycoplasma suis disease prevention and treatment.This study choose the most useful traditional Chinese medicine,with research confirmed that both antibacterial antiviral and insecticidal obviously effect of traditional Chinese medicine, Application of mycoplasma suis in vitro culture and real-time PCR technology, evaluation traditional Chinese medicine inhibit case of mycoplasmasuis. Traditional Chinese medicine for mycoplasma suis inhibition. Lay the foundation for the prevention of mycoplasma suis disease. The results showed that:1. As a carrier in red blood cells in vitro with RPMI1640medium mycoplasmasuis, the extension of incubation time, M.suis is constantly increasing, the number of high-speed centrifugal M.suis proliferation number than low speed centrifugal M.suis proliferation.2.4000μg/mL single and compound Chinese medicine with erythrocyte adhesion mycoplasmasuis were cultured in vitro, training60h to mycoplasmasuis has certain dissociation effect. Dissociation effect: Sophora70%, Areca Catechu65%, Chinese Herbal Compound1was85%, Chinese Herbal Compound3was81%, Chinese Herbal Compound5was74%.3.4000μg/mL single and pound Chinese medicine for60h cultured in vitro with mycoplasmasuis negative Erythrocyte. Adhesion effect:Sophora76%, Areca Catechu75%, Radix Dichroa73%, Chinese Herbal Compound1was79%, Chinese Herbal Compound3was76%, Chinese Herbal Compound5was75%.4.4000μg/mL single and compound Chinese medicine first with mycoplasmasuis cultured in vitro, Then add the negative Erythrocyte were cultured in vitro for60h, Traditional Chinese medicine inhibition mycoplasmasuis for reducing, mycoplasmasuis adhesion of Erythrocyte for reducing. Decrease effect: Sophora78%, Areca Catechu77%, Radix Dichroa74%, Chinese Herbal Compound1was82%, Chinese Herbal Compound3was80%, Chinese Herbal Compound5was76%.5.4000μg/mL single and compound Chinese medicine first with negative Erythrocyte15h, Then add mycoplasmasuis cultured in vitro for60h, with the negative adsorption capacity of red blood cells to prevent mycoplasmasuis stronger. Prevent effect:Sophora71%, Areca Catechu64%, Chinese Herbal Compound1was30%.