Research On The Effect Of Oregano Oil And Main Components On Rumen Fermentation Characteristics And Methane Production

This test analyzes the main wild oregano oil component vitro(In Vitro) test were evaluated the Mechanism of oregano oil and its major components thymol, p-cymene on ruminal fermentation and methane production. The paper is divided into two parts:Part1. To explore the effect of wild oregano oil on rumen fermentation characteristics and methane productionExpl. The study on effect of different doses of Oregano oil distillation extraction (0、24、48and72mg/90mL) on rumen fermentation and methane eminssion of cattle in vitro12-h batch culture of rumen fluid with a60:40concentrate:forage diet, the aim was to select suitable oregano oils of supplement.Part2. ffect of the main components of oregano oils on rumen fermentation characteristics and the influence of methane productionExp2. The qualitative and quantitative determination of oregano oil using Gas chromatography-mass spectrometry (GC-MS).Exp3. The study on effect of different doses of thymol(0、11、22、33and44mg/90mL) on rumen fermentation and methane eminssion of cattle in vitro12-h batch culture of rumen fluid with a60:40concentrate forage diet, the aim was to select suitable thymol of supplement.Exp4. The study on effect of different doses of p-cymene (0、20.6、27.5、34.4and41.3mg/90mL) on rumen fermentation and methane eminssion of cattle in vitro12-h batch culture of rumen fluid with a60:40concentrate:forage diet, the aim was to select suitable p-cymene of supplement.Exp5. The study on effect of thymol and/or of p-cymene (control,0mg/90mL; thymol,33mg/90mL;p-cymene,20.6mg/90mL;thymol+p-cymene,33+20.6mg/90mL) on rumen fermentation and methane eminssion of cattle in vitro12-h batch culture of rumen fluid with a60:40concentrate:forage diet, the aim was to select suitable thymol and of p-cymene of supplement.The results were as follows:(1) Certain concentration range of oregano oil can regulate rumen microbial fermentation,24mg/90mL oregano oil can increase the total volatile fatty acids (TVFA), the test groups were added oregano oil to improve the final pH value of the culture solution, reducing the final state of ammonia nitrogen concentration,48,72mg/90mL oregano oil reduces methane production.(2) Wild oregano oil extraction, gas chromatography-mass spectrometry (GC-MS) for qualitative determination of oregano oil was the main component of thymol and p-cymene, the ratio is about1.6, the main qualitative composition of oregano oil is thymol (46.38%),p-cymene (28.84%) and γ-terpinene (7.74%); the quantitative determination of oregano oil results of44.52mg/g thymol,27.69mg/g p-cymene.(3) Relative to controls, it seems to be more intense for the effect of thymol,22mg/90mL level would significantly reduce TVFA and methane production; p-cymene, at27.5,41.3mg/90mL, significantly increased the final pH, of the culture medium,34.4mg/90mL significantly reduces the ammonia N concentration.(4) The study shows that a specific ratio of thymol and of p-cymene influence on rumen microbial fermentation to produce a synergistic effect.33mg/90mL thymol,20.6mg/90mL of cymene regulation of rumen microbial fermentation, and the two had a synergistic effect on rumen microbial fermentation.