| The experimental aim was to explored the effect of methane inhibitors on rumen fermentation and nutrition metabolism of sheep in vitro and in vivo. Firstly, the methane inhibitors were fumaric acid, allicin and fumaric acid, different doses of the inhibitors were screened through batch culture in vitro. Secondly, 16 wethers with goodbody condition, similar weight were selected, rumen were cannulated permanentally for studying different methane inhibitors affect to rumen fermentation, nutrient metabolism in vivo.The first section, six wethers which were similar weight(28±1.0kg), good body condition and with permanent rumen fistula were selected as rumenant liquid donor. Different levels of the inhibitors were designed as fumaric acid(30mg, 40 mg and 50mg), allicin(100mg, 300 mg, 500mg), anthraquinone(10mg, 20 mg, 100mg) in per kilogram basic dietary respectively. Samples of culture fluid were collected at 3, 6, 9, 12 and 24 h, the pH, ammonia nitrogen(NH3-N), bacterial crude protein(BCP), methane(CH4) and VFA were determined as for comparing inhibitors parameter and screening a suitable dose. The results showed that different doses of fumaric acid, anthraquinone, allicin in diet had no significant affected on pH(p>0.05); the BCP(p<0.05), ammonia nitrogen(p<0.05) and propionic acid(p<0.05) concentration were increased significantly by added fumaric acid 40 mg, 300 mg of allicin and anthraquinone 20 mg, and the concentration of acetic acid and acetic acid/acrylic acid were reduced(p<0.05); the total gas volume in 12 h and 24 h were increased(p<0.05), and the methane yields were significant decreased(p<0.05).The second section, sixteen wethers which were good body condition, similar weight, and with ruminal permanental cannul were selected. The experiment were adopted as randomized block design, and divided into 4 groups with four sheep randomly, the control group fed as basal diet, the other three treatments were added 40 mg fumaric acid, allicin 300 mg, and 20 mg anthraquinone in the basic diet. Training and feeding periods were continued for 2 weeks repectively, and experiment period was about 3 days, the sample of sheep ruminal fluid were collected to determined pH, NH3-N, BCP, VFA and CH4 concentrations and nutrients metabolism. The results were as follows: compared with control group, supplemented 40 m g/kg fumaric acid, 300mg/kg allicin and 20mg/kg anthraquinone in the basic diet respectively, the rumen pH no significant influence(p>0.05) within 9h after feeding in sheep, and the pH of the allicin group was increased significantly(p<0.05); the rumen ammonia nitrogen concentration were reduced significantly after feed 6h(p<0.05), and the rumen BCP concentration after the 9h were increased significantly in 3 treatments, including at the highest of allicin group. There were the trended to reduced the acetic acid and acetic acid/propionic acid ratio and increased the propionic acid and total VFA(p>0.05). Compared with the control group, there were significant influence on rumen methane yield(p<0.05), the methane concentrations were decreased with 38.28% and 37.61% respectively, after supplemented garlicin and anthraquinone 6h.Compared with the control group, anthraquinone and allicin in the diet significantly affected to the energy metabolic rate(p<0.05), allicin groupwas the highest one; there were significant effect on the apparent digestibility of DM, OM, CP, NDF, ADF, Ca and P(p<0.05), allicin group was the highest one. Added garlicin in diet significant increased the dry matter intake and nitrogen digestibility(p<0.05).This test showed that the diet for sheep added to 300mg/kg garlicin agent was benefited to promoting the rumen fermentation, reduced methane production and improved the nutrient metabolic rate. |
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