Phylogenetic Analysis Of The Deltocephalinae Based On 28S RDNA Sequences

Deltocephalinae, the largest subfamily of Cicadellidae based on lots of described species, contains about 6200 species in 36 tribes distributes worldwide in habitats ranging from xeric grasslands and tropical raiforest. The group feed on the phloem sap of a wide variety of vascular plant and species ranges from monophagous to polyphagous. Deltocephalines is also an economically important group because it contains numerous vectors of agriculture plant pathogens. The recent morphological and molecular phylogenetic analyses of Cicadellidae and a morphology-based analysis of Deltocephalinae and related subfamilies indicated that several previously recognized cicadellid subfamilies are closely related to or derived from within Deltocephalinae, these analyses provided a comprehensive or well-supported hypothesis of the phylogeny of Deltophalinae. The 28S rDNA genome were extracted from 30 species of Deltophalinae and three outgroups are included. The sequences of 28S rDNA(650-750bp) have been acquired by PCR technique and sequenced using primers of Hemiptera. We analyzed genetic information using software with Clustal 2.0、MEGA 4.0、PAUP4.0 and reconstructed the phylogenetic relationships tree though Neighbor-Joing(NJ)、Maximum-Parsimony(MP)、UGMPA、MrBayes V3.0. The 28S data showed strong support for the monophyly of Deitocephalinae as defined here. The conclude as following:1. The average content of A、T、G、C of 28S rDNA gene sequence of 35 species of Deltophalinae are 20.5%、20%、28.1% and 31.3% respectively, and the contant of A+T is 40.5%, G+C content is 59.4% that the outcome show a big G+Cbias.2. The average of transition and transvertion ratio of 28S rDNA gene sequences is 1.814 and the transition is distinctly more over transvertion.3. The result of genetic distance shows: the smallest genetic distance between outgroup and ingroup is 0.04, the biggest is 1.56; the smallest genetic distance between ingroups is 0.02, and the biggest is 1.64.4. The evolution tree of parial Deltocephalini based on the NJ、MP、UPGMA mothoeds are unanimous which shows the more higher of the confidence level. The Bayesian tree has a lowconfidence level because of the nondispersed branch. 5. Goniagnathus separated firstly one from the cladogram form the most primitive tribe Goniagnathini of the group. The Phylogenic hypothesis suggests that Paralimnini and Deltocephalini form a monophyletic group; Scaphoitettix should transfer to Mukariini and Phlogotettix Ribautshould belong to Scaphoidelini; Drabescini should be including as a tribe of Deltocephalinae.