Optical System Design Of Four-channel Dual-laser Flow Cytometry

Flow cytometry is a combination of modern physics electronic technology, lasertechnology, computer technology in one of the advanced technology, within a secondof tens of thousands of biological cells or particles while doing multi-attribute,multi-parameter measurements.. Is a highly accurate recognition, rapid, highsensitivity detection methods of biological cell particles, It plays an extremelyimportant role in modern scientific and medical. Flow Cytometry is high-techequipment developed by Flow cytometry, has been widely applied to various fieldsof life and scientific research. However, the country currently has no one companyor research institutions developed with independent intellectual property rights of theproduct flow cytometry, flow cytometry in China’s development is almost blank.This paper mainly from the flow cytometer sheath flow systems, excitation lightsource with beam shaping system, the forward scattered light and side scatter lightdetection light path design and other aspects of flow cytometry fluorescence opticalsystem expand research and design.1、According to the requirements of the sample fluid and detecting requirementsof flow cytometer, Use of sheath fluid flow system focusing principle for the designof flow system, Let the sample stream made by cell wrapped in a sheath flowshoowed a single linear fluid flow through the laser detection region, and with theexcitation laser perpendicular to each other. Does not enable the cells to produceinterference between the scattered light and the fluorescence signals.2、According to the requirements of cell size and the width of the sample flowand the focus detection accuracy requirements, using zemax software design thebeam forming system, the laser light generated in the laser beam after the beamforming system is formed the spot of10μm×75μm elliptical, to meet the demandfor the detection of cells, and when the cells through the detection zone it would notaffect the optical signal detecting because of the beam is not too wide or otherreasons.3、According to the position and sizes of the silicon photocell and sizes, Use the optisworks software design the front and side scattered light detect optical path.Make the scattered light energy focused on the silicon photocell after the light gothrough the optical system to achieve the detection of the scattered light energy.4、Make the in-depth research of fluorescent classification and generation.Thenunderstanding the principles of fluorescent produced,and discussing the relationshipbetween fluorescent spectrum and excitation spectrum. Make the preliminary studyof the detection light path of fluorescent.