The Design Of A Portable Flow Cytometry Platform Based On A Microfluidic Chip

In the past two decades,flow cytometry has been widely used in medical disease diagnosis due to its ability to count,characterize and sort cells.As a powerful cell analysis tool,flow cytometry has been able to detect cells accurately and fastly.However,problems such as large size,high cost,and high consumption have become the main bottlenecks for the further development of traditional flow cytometry.In recent years,the rapid development of microfluidic technology has pointed out a new direction for the development of flow cytometry.With microfluidic technology,traditional chemical biological experiments can be performed on a microfluidic chip which has only a few square centimeters.The combination of flow cytometry and microfluidic can develop a set of portable and low-cost flow cytometry equipment based on the microfluidic technology.But at the current stage,the flow cytometer device based on the microfluidic chip is only miniaturized on the chip.Many peripheral components such as pumps,laser light sources,computers and microscopes are still very bulky and expensive.Therefore,it is difficult to use it in primary care.The research goal of this paper is to design and improve the sampling system,optical system and detection system based on the existing flow cytometer,and develop a truly portable flow cytometry detection platform.In this paper,a new driving method for electrokinetically-induced pressure-driven flow is designed to drive cells without the need for a huge pump.At the same time,laser diodes and photodiodes are used to replace the laser light sources and photomultiplier tubes respectively,which greatly improves portability and reduces costs.All parts are reasonably positioned to get better detection results.A microcomputer is used as a signal receiving and processing terminal,and a test program is written in Labview and Matlab to complete the functions of signal detection,signal processing,signal analysis,and signal storage.Finally,in order to further realize the portability of the detection platform,this paper also writes a signal detection and processing program based on the Android development platform,so that the signal detection terminal can be replaced by a smartphone and run away from the system,which has better portability and maintainability.The detection platform was verified through experiments.In the experiments,10μm,5μm,2μm and 900 nm fluorescent samples were detected,and all of them could get better detection results and better resolution.The detection platform can also perform dual-wavelength fluorescence detection and can simultaneously detect two samples,which also greatly improves the detection efficiency.Finally,the detection platform was integrated.The entire platform is small in size and highly portable.