Quantitative Analysis Of NMR Technology Piperine, Ibuprofen, Hydroquinone Isomers

With the continuous improvement of nuclear magnetic resonance technology, its application in various fields has become more and more extensive.Nuclear magnetic resonance has become an indispensable means in the structural analysis of the compound. Due to the continuous improvement of the sensitivity of the nuclear magnetic resonance instrument, so that the quantitative NMR has been greatly developed.Medicinal analysis is an important part of medicine, it is a method of chemical, biological, physical chemical and other methods to research the chemical structure and content. Strict control of drug quality is the basic guarantee for human health. And drugs often contain a isomerism phenomenon, the analysis of the isomer of medicine has an important significance.Nuclear Magnetic Resonance (NMR) technology plays an important role in the field of medicinal analysis. It can also analyze the structure and quantitative analysis of the drug at the same time.We chose three drugs that contain isomers, in order to explore the application of NMR technology in the isomer body of medicine.They are piperine, ibuprofen and hydroquinone,with cis-trans isomerism, chiral isomerism and Position isomerism.The structure of piperine was determined by 1D and 2D NMR, quantitative analysis of the content of the piperine base by using the 1H NMR.On the basis of this study, a new method for quantitative piperine content was studied.After comparison experiments show that the new method is simple and feasible. Ultraviolet radiation of piperine, to analyze the structure of piperine using 1D and 2D NMR technology. To study the relative content of the piperine isomers at different radiation time.The structure of ibuprofen was analyzed by NMR technique.Using chiral sulfocarbamide was taken as chiral solvent reagent, identification of ibuprofen enantiomers under the action of DMAP.Analysis of the attribution of (R)-(+)-ibuprofen and (S)-(+)-ibuprofen in 1H NMR.And simultaneous quantitative analysis of these two compounds.with NMR technology based on quantitative determination, establishment of content a method to determination of dihydroxybenzene isomers.The results of Catechol, Resorcinol, Hydroquinone is all the good linearity in linear range. This method has the advantages of high sensitivity, the method is simpler,reproducible.This method can be used for the quantitative determination of dihydroxybenzene isomers.