| Chimonanthus Praecox has good cold resistance with own unique aroma.Since ancient times,it has been used as a raw material of traditional natural flavors.In this article,we experiment with it as raw material.At room temperature with a weak base protein is extracted.Taking extraction rate of protein as an index to find the best process to extract protein.And on the basis of this condition we added glucoamylase to assist protein extraction. Similarly, taking the protein extraction as an index to get the best process.And,we take the extraction rate of essential oil as the index to get the best process essential oil.So that we can get the best process to extract essential oil.The experimental results are as follows:1. Protein is extracted in the case of room temperature with a weak base,taking the extraction rate of protein as an index.Four factors affecting the extraction rate of protein was as follows:solid-liquid ratio > extraction temperature > extraction time > alkaline p H.And when the solid-liquid ratio was 1:6,extraction temperature was 25 ℃,extraction time was 4h,alkaline p H value was 7.2,the best process was got,the extraction rate of protein was highest,reached 28%,the purity of protein was 68%.2. Adding glucoamylase to assist extract protein on the basis of condition 1st.Similarly,taking the extraction rate of protein as an index.Five factors affecting the extraction rate of protein was as follows:solid-liquid ratio > glucoamylase action time > alkaline p H > glucoamylase action p H > the amount of glucoamylase.And when the solid-liquid ratio was 1:6,glucoamylase action time was 30 min,alkaline p H was 7.2,glucoamylase action p H was 4.0,the amount of glucoamylase was 0.03%,the best process was got,the extraction rate of protein was highest,reached 31%,the purity of protein was 73%.3. Taking the extraction rate of essential oil as the index,getting the best process of steam distillation of extraction of essential oil.Four factors affecting the extraction rate of essential oil was as follows:ion concentration > solid-liquid ratio > steam flow > distillation time.And when the ion concentration was 2%,solid-liquid ratio was 1:10,steam flow was 700 ml,distillation time was 210 min,the best process was got,,the extraction rate of essential oil was highest, reached 0.15%. |
