| Mushrooms(Lentinula edodes), also called Yuuna Ka, Flower Mushroom and Shannon bacteria, mushrooms is edible fungi. It belongs to the Fungus, Basidiomycetes, Agaricales, Tricholomataceae, genus of mushrooms. Modern research shows that the edible mushrooms have enhance the role of immunity, mushrooms polysaccharide is the effective components of mushrooms. At present, the extraction of mushrooms polysaccharide most use water extraction and alcohol precipitation, in this paper, taking the technology of submerged fermentation to mushrooms, removing impurity of the fermentation liquid, and prepared into oral liquid to improving body immunity.In this paper, the main research contents are:(1) the preparation of mushrooms submerged fermentation liquid;(2) study on the preparation processing of mushrooms polysaccharide oral liquid;(3) the establishment of mushrooms polysaccharide oral liquid quality standards;(4) investigate on the stability of mushrooms polysaccharide oral liquid. Main research results are as follows:(1) The preparation of mushrooms submerged fermentation liquid: the fermentation medium is glucose 20g/L, yeast powder 5g/L, KH2PO4 1g/L, MgSO4 0.5g/L, pH for natural, the volume of liquid is 4L, inoculation is 10% and culture in the5 Lfermentor,Temperature 27 °c, 150r/min speed, ventilation initial 5L/min, to incr easing the ventilation volume 7L/min after 3-4d, at the late stage of fermentation, pH dropped to under 4.0, to maintain the pH value is constant by adding alkalescent solution automatically. Determination the content of polysaccharide in fermentation liquid is 74.503mg/L by the method of phenol sulfuric acid.(2) The study on the preparation processing of mushrooms polysaccharide oral liquid: removing impurity of the fermentation liquid with the natural clarifying agent —ZTC1+1III, the adding sequence is adding component B first,then adding component A, the dosage of clarifying agent viscose glue is 4%, diluted the fermentation concentration to 80%, the temperature is 60℃, stirring speed is 200 r/min, pH 5.5. The adding amount of preservatives potassium sorbate is 0.2%, flavoring agent stevioside is 0.07%, sieve with 100 mesh. Then make the mushrooms fermentation filling in the 10 mL glass bottle after high temperature and high pressure sterilization, sealing.(3) The establishment of mushrooms polysaccharide oral liquid quality standards: Liquid relative density of 1.0617 or above, oral liquid with pH value of 5.5, microbial limit meet China Pharmacopoeia 2010 "(a)" Appendix III of microbial limit, the content of polysaccharide is 74.545mg/L.(4) Investigate on the stability of mushrooms polysaccharide oral liquid: Should fill in the brown bottle and store away from light. Temperature can not be considered, because it is not the significant factor to affect the stability of oral liquid. The accelerated test results of mushrooms polysaccharide oral liquid shows that the traits and pH values of mushrooms polysaccharide oral liquid is not sensitive to temperature, and the clarity also did not produce significant changes. It not becomes turbid in the detection of samples. So, the stability condition of mushrooms polysaccharide oral liquid is good. |
