| Jasminum nudiflorum Lindl always were mad use of leaves and flowers as a kind of medicine. In this paper, the different extraction were choose to extract the total flavonoids in jasmine. Within the response surface method, the optimal extraction conditions was got to optimize the ultrasonic extraction process, and by antioxidant activity model, ethyl separate were screened for antioxidant activity to find the strongest parts of the site, then tesed its anti-aging test. The silica gel column chromatography and high-speed countercurrent chromatography method were used to separate compounds in the parts of ethyl acetate, which had isolated four flavonoids, and carried on the determination of antioxidant activity. The experimental results were as follows:1. Compared the soxhlet extraction with ultrasonic assisted method, soxhlet extraction was 1.98%, and ultrasonic assisted extraction was 3.15%.2. Ultrasonic method had be optimized the extraction conditions of flavonoids extraction rate through the response surface method, and extract conditions were ethanol concentration, solid-liquid ratio, temperature and time as the independent variable, which had obtain the best extraction conditions as follows: 68% ethanol concentration, solid-liquid ratio, all the sons of 40 min extraction time, extraction temperature 50℃. In this condition, the total flavonoids extraction yield was 3.15%, the little difference between the theoretical value of 3.21%.3. Each phase extraction was determined by the flavonoids content, the conclusion was : ethyl acetate > n-butanol phase > total phase > chloroform > petroleum ether. Secondly, the extraction phase and the total components were tested on the four index to analysis the antioxidant activity of DPPH, hydroxyl radical and superoxide anion radical scavenging and total reducing power.4. Ethyl acetate was studied on the mouse induced by D-galactose for aging test, and it was found that ethyl acetate phase flavonoids could improve the activity of SOD, CAT, POD and GSH in serum and skin tissue of mouse, and also inhibit the content of MDA and Hyp in serum and skin tissue of mouse.5. Using the traditional silica gel column chromatography and advanced high-speed countercurrent chromatography method, the flavonoids in primrose, ethyl acetate phase were for further purification, in the silica gel column chromatography with chloroform/methanol as eluent, in high speed countercurrent chromatography with chloroform/methanol/water separation system, the results were showed 4 different monomer compounds, while two of them were new compounds, using 1H-NMR, 13C-NMR, HMBC, MASS spectroscopy technology on the structures of compounds, respectively apigenin(Y3-5), 6’-O-(3,5-O-dimethylgalloyl) quercetin 3’-O-β-D-glucopyranoside(Y4-8), luteolin(Y4-9-2),6’-O-(3,5-O-dimethylgalloyl)3,5,7,3’,4’,5’-hexahydroxyflavone3’-O-β-D-glucopyranoside(Y4-11-1). And 6’’-O-(3,5-O- dimethyl-galloyl) quercetin 3’-O-β-D-glucopyranoside(Y4-8), 6’-O-(3,5-O-dimethy-lgalloyl)3,5,7,3’,4’,5’-hexahydroxy-flavone3’-O-β-D-glucopyranoside(Y4-11-1) are new compounds.6. DPPH method was developed for the determination of monomer compounds in vitro antioxidant activity. Y3-5, Y4-8, Y4-11-1 had the capacity to remove DPPH free, which could reach 90% and be more than the control of VC. The study showed that Y3-5, Y4-8, Y4-11-1could owe very strong ability for antioxidant. At the same time with the monomer compounds to the D-galactose induced aging test in mice, it showed that Y3-5, Y4-11-1 could improve the activity of SOD, CAT, POD and GSH in the mouse’ serum and skin tissues, on the other way, SOD, CAT, POD and GSH could inhibit the content of MDA. This showed that Y3-5, Y4-11-1 had a certain inhibitory effect against aging. |
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