Study On The Permeability Of Plant Seed Cell Membrane By Ultrasonic Irradiation

Physical agriculture has been participated by the technology information and physical theory in the development process since20th century, which applied in agriculture by means of the physical theory and the application of science and technology, which can well improved the overall efficiency of agricultural production and increase crop yield. As an important branch of agriculture biophysics, Ultrasound breeding plays an important role in the history of the development of agricultural science and technology. It has the features of low cost, high performance and efficiency, and environmental protection.From the theoretical aspects of the mechanism of ultrasonic radiation, the mung seed breeding are described in this paper. By using the ultrasound characteristic parameters, we designed some experiments to discuss the mung bean seeds cell’s membrane permeability with the treatment of ultrasound, and explained the reasons which the germination rate are improved, and the germination cycle are shortened by using the ultrasound. There is a certain reference value for applications in agriculture by using of ultrasound.Based on the combination of theory and experiment on each other, we can draw the following conclusions:(1) Through the establishment of cell membrane vibration model, the theoretical calculation of the resonance frequency of the mung bean seed’s germ cell membrane. Through experiments, theory and practice were matched. It explained that the resonance frequency of the ultrasonic is as same as the frequency of the seed cell, which can induce the resonance of the membrane, so that the maximum energy of the sound wave can get into the cell with resulting in a series of biophysical effects. Breeding experiments with the ultrasonic frequency are basically consistent with the theoretical optimum frequency, thus we can confirm the validity of the theoretical model.(2) By calculating the optimal frequency of a certain seed with the equation which is radiated with the ultrasound, it can be more targeted in the design of the transducer; while in the process of ultrasonic breeding, due to the lack of a center frequency, the experiences blindly rely on experiments, will increase in workload. Through the data which are obtained by experiments on the basis of the size of the cell, we calculated the center frequency of ultrasound breeding, and thus reduced the workload of the experiment, and reduced the experimental difficulty. It had some more practical significance on the ultrasonic breeding.(3) To some extent, the length of germination time may reflect the influence of ultrasonic radiation on mung bean seed germination. The shorter germination time can indicate that the ultrasonic radiation has greater the influence on the green beans. In this paper, we designed experiments, with the conditions of ultrasonic frequency, power and radiation time respectively, to discuss their respective effects on mung bean seed germination, and got some experimental results.(4) Through the two-factor recombination experiments, we can conclude that the frequency of ultrasonic radiation has the maximum impacts on plant germination rate and germination potential, the radiated power has less effects on the plants, and followed by the effects of radiation time.