Basic Reserach On Application Of Screen-printed Method For Microfluidics Dielectrophoresis Chip Preparation

Microfluidics dielectrophoresis chip can manipulate and separate biological materials(such as cell, DNA, protein) basing on the principle that distinctive dielectric property resultsin distinctive field force in inhomogeneous field. This paper put forward to makedielectrophoresis chips by screen-printing method,which is a mature technology in industryfield.It aims to solve the existing problems in production such as high cost, long circle,difficulty to industrialize.This paper first generally illustrates current academic efforts on the research ofdielectrophoresis chip and gives a brief introduction about the theory and formulas aboutdielectrophoresis. Meanwhile the advantages of screen-printing method are discussed.Then it focuses on the production process of the chip, including preparing microelectrode by screen-printing method, preparing micro-channel by photolithography,sealing,punching etc. After that,some good measure was proposed to improve theproperty.The effects of different factors exerting on the screen-printing was analysed.Inaddition,the advantage and disadvantage of the screen-printing and photolithography wascompared.When the preparation of the chip is finished, Comsol is used to analyze the distributionof electric field intensity surrounding castellated electrode and parallel electrode, themagnitude and direction of dielectrophoresis forces on different spot and to predict thedistribution of particles. PS microparticles and yeasts are deployed respectively to conductdielectrophoresis experiments in order to measure their dielectric properties under differentfrequency and solution conductivity, preparing for the separation procedure.The last part of the paper presents a brief analysis on the theory of the separation andexplains in detail how frequency, solution conductivity and flow rate can affect the efficiencyof the separation, finding an optimal condition to successfully separate these two particles.To sum up, the paper provides theoretical support and experimental basis for thescreen-printing method of the production of microfulidics chip.