The Effects Of Simulate Alternate Hypoxic Training On Rat Mitochondria Respiratory Chain Complexes

Simulated hypoxic training is a new scientific training method that is developed in recentyears, it plays a positive role in sports training. The application of artificial hypobaricchamber simulated altitude training methods, to establish animal model of Fixed Altitude2500m,3500m alternating hypoxia training, Study on different altitude and effects ofalternative training on skeletal muscle mitochondrial respiratory chain function in rats.Discussing the dynamic changes of the activity of the respiratory chain in mitochondria ofskeletal muscle at different altitudes alternating hypoxia stimulation, which could provides areference for the research of choosing the proper training altitudes and the research of hypoxicadaptation.experimental methodThis experiment selected healthy male Wister rats aged60, February, weighing about130g, provided by Lanzhou Institute of traditional Chinese medicine, screening according tothe animal adaptability,50retained rats were randomly divided into5groups. These rats weredistributed to Normoxic quiet control group(C), Normoxic training group(E),2500mhypoxiatraining group (2500E),3500mhypoxia training group(3500E), Alternating hypoxia traininggroup (AE), each of them got10,then training. Normoxic training based on1500m ofLanZhou, give the rats four weeks normal oxygen increasing load training by level of animaltreadmill. Wnen Hypoxia training, the oxygen tension were15.4﹪and13.6﹪separately. it isequivalent to the altitudes about2500m and3500m. the training groups of2500m and3500m,both stay at their altitudes for live and trainning. While the Alternate training group got sixdays training every week (the fore there days stay at2500m, the spared days stay at3500m),one day rest(at2500m). Draw materials after all the rats finished the last training andgot a48hours normoxic recovery. Determine the weight, bloods index and the activity of theSkeletal muscle mitochondrial respiratory chain enzyme complexesexperimental result:four weeks training make an obviously weight developing to all the groups.Compared with Normoxia quiet group, the growing level of the training group have asignificant difference, put up a downtrend, while the Hypoxia group has no significantdifference by comparison, it means longtime training has effect on body weight, hypoxia hasno big negative effects. Because of the different environment and training methods, hypoxia group get a small lower weight than normoxia, maybe the hypoxia stimulation made thetraining consume more fat, then the fatting weight increase relatively, causing the organismcomposition change. Alternating hypoxia group has little difference with other hypoxiagroups.2.compared with the quiet group, each groups’ hemoglobin contents have obviousdevelopment (P<0.01,P<0.05), have a rising trend. Normoxic training group(E),2500mhypoxia training group(2500E),3500mhypoxia training group (3500E), Alternatinghypoxia training group(AE) improved by1.46％、7.70％、9.69％、12.68％respectively..compared with Normoxic training group, each hypoxia training group’shemoglobin contents have obvious development (P<0.01), each improved by （2500E）6.15％、（3500E）8.11％、（AE）11.06％, Alternating hypoxia training group(AE) both got anobvious development（P<0.01）, impoved by4.62％compare with2500E,and3.98％with3500E.3500E and2500E make a comparison, both have little difference, but have a fixedrising trend. RBC has the similar change rule to Hb.3. compared with the normoxic quiet group, the rats in E group, whose activity of theSkeletal muscle mitochondrial respiratory chain enzyme complexes III had significantimprovement (P<0.05), improved by50％, the activity of enzyme complexes II、III、IV hadno significant improvement, but presented a fixed rising trend, improved by28.05％、6.12％、12.20％respectively. It means longtime training can improve the activity of the Skeletalmuscle mitochondrial respiratory chain enzyme, improve the aerobic capacity.compared withthe2500E group, group AE the activity of enzyme complexes I、II、III、IV all have a fixedincrease, improved by61.9％、46.15％、34.78％respectively. When compared withgroup3500E, I、II、IV have a fixed increase, improved by6.92％、13.43％、8.77％. but allthe hypoxia training group compare with normoxic training group and groupAE compare withgroup3500E, enzyme complexes III both had a fixed downtrend.Conclution:1.Exercise training can restrain the rat body weight, reduce the amplification of bodyweight, and the training load and hypoxic stimulation had little effect on the body weight ofrats.2.Normoxic training can improve the Oxygen transport capacity, but hypoxia trainingcan do better, and alternating hypoxia training do the best. 3.Normoxic training could improve the capacity of the Skeletal muscle mitochondrialrespiratory chain, but hypoxia training could make the capacity improve farther, as threehypoxia training pattern in this experiment, alternating hypoxia training had a more obviouseffect.