| By monitoring BLA thick after exercise buildingintermittent high intensity exercise model Combinationexercise and hypoxia, probe the effect of hypoxia or/andexercise on rats weight, muscle glycogen content, SDH activity,LDH activity and action of HIF-1α, dowse the correlation ofSDH and LDH activity and HIF-1α.Methods: fifty healthy male adult Sprague-Dawley rats arerandomly divided into five groups: the normorxic group (N,n=10),the hypoxic groups(H, n=10),the normorxic exercise group(NE,n=10), normoxic exercise and hypoxic living group(NH,n=10),hypoxic exercise and living group (HH, n=10), adding up to 8weeks. After the experiment, all of the rats were killed afteranesthesia, took blood of 3 ml, one piece of gastrocnemius wastook out to made into tissues homogenate fluids, the other wasmade into paraffin section. Test the Hb,Hct,RBC in the blood;LDH, SDH activity and muscle glycogen content in the muscletissues, HIF-1αprotein content in the paraffin section.Results:(1)The blood lactic acid in rats is about 12mmol/L, anddifference isn't significant, which is accordance with therequirement of intermittent high intensity training.(2)There is a significant difference in NH/HH group thanthe normoxic in the HIF-1α(p＜0.05, p＜0.05)(3)There is an increasing trend and a significantdifference (p＜0.01, p＜0.001) in H/NE/NH/HH group than thenormoxic in muscle glycogen content. There is increasing120.4%,124% and a significant difference (p＜0.01, p＜0.01) inthe NH/HH group than the hypoxic in muscle glycogen content.(4)There is lighter in the NE/NH/HH group than the normorxic group in weight, the difference has the significance.(p＜0.05, p＜0.01, p＜0.05).the weight is lighter in the NH/HHgroup than the hypoxic group (p＜0.05, p＜0.05)(5)muscle glycogen content in H/NE/NH/HH group increased,a significant difference (p＜0.01, p＜0.001), compared with thenormoxic. The SDH activity in the NH/HH group increased 72.6%,73.0%, a significant difference(p＜0.05, p＜0.05) compared thanthe hypoxic group.(6) The related coefficient is 0.500 between SDH activityand muscle glycogen content(p＜0.01).(7)The LDH activity decreased trend from the hypoxic groupto the HH group. No significant difference among the groups.Results:(1)By detecting BLA, adjust and control training intensitybetter. All the training belongs to reinforcing intermittenttraining.(2)The intermittent training lowers the RBC/Hb/Hctremarkably, which perhaps is the reason of heavy intensitytraining resulting in cell hemolysis.(3)Intermittent and hypoxic training have weight-losing toa certain extent and pure hypoxic also have this trend, but notobviously.(4) Hypoxic training can increase significantly HIF-1α,but only hypoxia or training can't. Intermittent training orHypoxic training can both alleviate the weight of rats and theHN group is the best obvious.(5)Both hypoxic and/or intermittent can increase glycogen,but only hypoxic is not as distinct as intermittent or hypoxictraining. In addition, both hypoxic and/or training canincrease aerobic metabolism capacity. Glycolitic capacity ofall experimental groups shows a decreasing trend, which perhapsis related with muscle types and detecting time. (6) HIF-1αinhibits glycolitic capacity, whose reason isnot obvious. Aerobic metabolism enzyme advances glycogenreserve; HIF-1αadvances aerobic metabolism capacity; aerobicmetabolism capacity which perhaps is related with manyhypoxic regulation genes. |
