Effects Of Ghrelin On Repeated Exhaustive Exercise Induced Systematic Inflammatory Response In Rats

Objective: to study the Ghrelin in repetitive exhaustion exercise induced the role of systemicinflammation in rats.Methods:43healthy male Wistar rats (300±10g) were randomly divided into four groups,sedentary group (R, n=6), repeated exhaustive exercise group(E, n=18), Ghrelin group(G,n=6)and repeated exhaustive exercise+Ghrelin group (GE, n=13).Rats ran to exhaustion three times daily for ten consecutive days at24m/min at a10%grade inthe E and GE group. The rats in the G group and GE group were injected with Ghrelin at a doseof8nmol/kg twice each day for10days. All experimental rats were killed at24h after the lastexhaustive exercise of tenth days. With ELISA kit analysis of LPS in serum and D lactate, TNFalpha, il-10, IL-6and HMGB1levels, with Western bloting method determination of heart,liver, lung, kidney, intestine, skeletal muscle in the beta, TNF, IL-1-alpha, IL-4, il-10, il-6,HMGB1protein expression.Result:1,LPS and D-lactic acid: Ghrelin and repeated exhaustive exercise had significant interaction(P<0.01). The content of LPS and D-lactic in the serum of group E was significantly higher thangroup R (P<0.01). The content of LPS and D-lactic in the serum of group G had no significantdifference compared with group R. The content of LPS and D-lactic in the serum of group GEwas significantly lower compared with group E (P<0.01).2, TNF-α, IL-6in the serum and IL-1β, TNF-α in tissues: Ghrelin and repeated exhaustiveexercise has significant interaction (P<0.01).The content of TNF-α, IL-6in the serum and IL-1β,TNF-α in tissues in group E was significantly higher than group R (P<0.01). The content ofTNF-α, IL-6in the serum and IL-1β, TNF-α in tissues in group G had no significant difference compared with group R. The content of TNF-α, IL-6in the serum and IL-1β, TNF-α in tissuesin group GE was significantly lower than group E (P<0.01).3, IL-10in the serum and the IL-4and IL-10in tissues: Ghrelin and repeated exhaustive exercisehas significant interaction,in IL-10in the serum (P<0.01). The content of IL-10in the serum inE group was significantly higher compared with group R (P<0.01).The content of IL-10in theserum in group G had no significant difference compared with group R. The content of IL-10inthe serum in group GE was significantly lower compared with group E (P<0.01). In the IL-4andIL-10in tissues, except the IL-4in the lung, Ghrelin and repeated exhaustive exercise had nosignificant interaction. In the IL-4in lung tissue,Ghrelin and repetition of exhaustive exercisehas significant interaction (P=0.044<0.05). The content of IL-4in lung tissue in group E wassignificantly higher compared with R group (P<0.01). The content of IL-4in lung tissue in groupG had no significant difference compared with group R. The content of IL-4in lung tissue ingroup GE had no significant difference compared with group E.4, HMGB1: HMGB1in the serum and tissues of Ghrelin and repetition of exhaustive exercisehas significant interaction (P<0.01).The content of HMGB1in group E was significantly highercompared with group R (P<0.01), The content of HMGB1in group G had no significantdifference compared with group R.The content of HMGB1in group GE was significantly lowercompared with group E(P<0.01).Conclusion:1. The repetitive exhaustion movement leaded to intestinal mucosal injury in rats, and alsoresulted in the rats with systemic inflammatory response.2. The Ghrelin could protect intestinal mucosa injury caused by repetitive exhaustion exercise ofthe rat.3. The Ghrelin restrained early inflammatory cytokines in rats in the earlier systemicinflammatory response.4. The Ghrelin restrained the inflammatory factor HMGB1in the later systemic inflammatoryresponse of rat.