Effects Of Ghrelin On Repetitive Exhaustive Exercise Induced Multiple Organ Dysfunction In Rats

Objective: To investigate effects of ghrelin on repetitive exhaustive exercise inducedmultiple organ dysfunction in rats.Methods:43healthy male Wistar rats (300±10g) were randomly divided into fourgroups, sedentary group (R, n=6), repeated exhaustive exercise group(E, n=18),ghrelin group(G, n=6)and repeated exhaustive exercise+ghrelin group(GE, n=13).Rats ran to exhaustion three times daily for ten consecutive days at24m/min at a10%grade in the E and GE group. The rats in the G group and GE group were injectedwith ghrelin at a dose of8nmol/kg twice each day for10days. All experimental ratswere killed at24h after the last exhaustive exercise of tenth days.Result:1. Compared with R group, body length, perinephrit and epididymis fat content,weight and the BMI value of rats in the E group and GE group were significantlyelevated; In the G group, body length and perinephrit and epididymis fat content,significantly increased, BMI decreased, body weight gain but no significant difference;Compared with E group, body weight, body length, perinephrit and epididymis fatcontent of rats were increased significantly in the GE group, and there was nosignificant difference in BMI value.2. Each organ and tissue morphology of rats were normal in the R group and G group;Liver, lung, small intestine and skeletal muscle injury of rats were serious, andmorphology changed obviously in the E group, there was no obvious morphologicalabnormalities in heart and kidney; Liver, lung, small intestine, skeletal muscle injurywere partly slighter in the GE group than E group.3. Compared with R group, in the E group and GE group，the CK activity，CKMB activity，ALT activity，BUN content and BUN/Cr value were significantly increasedin serum of rats, there was no significant difference in Cr content;In the G group,there were no significant difference in the CK activity, CKMB activity, ALT activity,BUN content, Cr content and BUN/Cr value; Compared with E group, ALT, BUN,BUN/Cr values were decreased significantly in serum of rats,and CK, CKMBactivity and Cr value were no difference significantly.4. Compared with R group, in the E group and GE group, blood leukocyte, monocyte,neutrophil count and percentage were significantly increased, and lymphocyte countand percentage decreased significantly in rats; In the G group, there were nosignificant difference in the blood leukocyte and classification of rats. Compared withE group, in the GE group, blood leukocyte, monocyte, neutrophil count andpercentage were significantly decreased, lymphocyte count and percentage increasedsignificantly in rats.5. Compared with R group, in the E group and GE group, small intestinal mucosathickness and villus height decreased significantly; in the G group, there were nosignificant difference in the small intestinal mucosa thickness and villus height.Compared with E group, in the GE group, small intestinal mucosa thickness and villusheight increased significantly.6. Compared with R group, in the E group and GE group, the serum DAO activitydecreased significantly in rats; in the G group, there were no significant difference inthe serum DAO activity. Compared with E group, in the GE group, The serum DAOactivity increased significantly in rats.7. Compared with R group, in the E group, MPO activity increased significantly in theheart, liver, lung, small intestine, skeletal muscle tissue, and there were nosignificantly difference in kidney tissue; In the GE group, MPO activities of liver,skeletal muscle tissue were increased significantly, and MPO activities of the smallintestine, lung tissue were decreased significantly, and there were no significantdifference in heart, kidney; In the G group, there were no significant difference inMPO activity. Compared with E group, in the GE group. MPO activities of the heart,liver, small intestine and skeletal muscle tissue were decreased significantly, there was no significant difference in the kidney tissue.Conclusion:1. Ghrelin reduces loss of body composition induced by repetitive exhaustive exercisein rats.2. Ghrelin ameliorates the small intestine, lung, liver hematological system andimmune system dysfunction resulted by repetitive exhaustive exercise in rats.3. The protective effect of ghrelin in repetitive exhaustive exercise rats may be relatedto improve the intestinal mucosal injury and anti-inflammatory effects.