| Objective:Exercise to lose weight as a key success factor is the loss of appetite and food intake after exercise changes. This study aims to study the impact of obesity in the rat hypothalamus and plasma Ghrelin after long-term aerobic exercise, and then study appetite and food intake variation, under a long-term study of aerobic exercise load changes on rat Ghrelin and 2, Ghrelin 2,4,6 weeks and each group of mutual relations, activities and food intake in rats and its mechanism were discussed between 4 and 6 weeks of each group. Reveal the variation of aerobic exercise on the body and the mechanism of action of Ghrelin Ghrelin, in order to develop and improve more scientific and reasonable exercise weight loss plan laid to provide a theoretical basis.Research Methods:The rat model of obesity:Buy female rats meet the test requirements from the Experimental Animal Center of Military Science 220,weighing 180-200g, randomized adaptive feeding after 1 week.Divided into general control and high fat diet group, the control group received normal diet ordinary;fat diet group was given a high-calorie diet. Each group feeding six weeks, eating and drinking freely into the record daily food intake and body weight and body length change weekly. According to the weekly measurements of body weight and body length, calculated Lee’s index, were randomly divided into a control group and a high-fat diet group according to Lee’s index screening modeling success obese rats, did not meet the requirements for the resistance group. Obese rats after modeling in addition to the remaining fat in the control group were fed with normal diet. Diet group and the resistance group after three days adaptive swimming, conducted daily 60 minutes of swimming,training six weeks, six days a week. Diet group continues from the previous two weeks to the normal diet of 80 percent to three weeks to the normal diet of 70% to the end of experiment. Each set of experiments were performed 6 weeks after the end of the movement immediately drawn and drawn three hours in the control group were also taken from the appropriate number of weeks. Experimental animal movement pattern:high 100cm, diameter 50cm plastic bucket allow rats to swim, exercise time rats one hour a day on Sundays. Motion cycle of 6 weeks.Sample Test:by radioimmunoassay staining of rat tissue in obesity and serum Ghrelin levels tested.Results:1.Two weeks Serum Ghrelin levels in the diet up to 3h group, followed by two weeks of normal control group, the least is the dieting group instantly. The average content of two weeks in each gastric tissue Ghrelin value marginally. The highest levels of obesity in the control group, the minimum content is instantly dieting group.2.4 weeks stomach tissue Ghrelin is the highest average levels of obesity in the control group, the content of the lowest resistance immediate group.3. Gastric tissue in six weeks, the average content of each group Ghrelin modest ratio variation, where in the content of the highest resistance to six weeks immediately set, the lowest content is 6 weeks obese control group.4. In the serum resistance 3h group,2,4,6-week average Ghrelin levels of aerobic exercise with the extension of the time with a gradual upward trend, that the average content of Ghrelin and aerobic exercise time is a positive correlation.3h gastric tissue resistance group, the average content of between 4 and 6 weeks Ghrelin is changed slight, from high to low phenomenon, there are some differences between the three V-shaped trend.But overall for the high, low, low.5. Diet 3h mean serum Ghrelin levels and 6 weeks of aerobic exercise cycle is a negative correlation.There was a significant difference (P<0.05) between two weeks and four weeks the groups six weeks.4 weeks there was no significant difference between 6 weeks.Dieting 3h group gastric tissue Ghrelin is the highest average levels of 6 weeks and 6 weeks showed a V-line trend, but overall for the Low,low,high, two weeks and four weeks was not significantly different.There was a significant difference (P<0.05) between 2 and 4 weeks and 6 groups.6. Serum Ghrelin six weeks on a diet immediately set the highestaverage levels were significantly (P<0.05) and the difference between 2and 4 weeks 6 weeks on a diet gastric tissue instantly set the highest average levels of Ghrelin is four weeks dieting instant group followed by 6 weeks, the minimum is two weeks, but the value is relatively small difference between 4 and 6 weeks.7. Obese control group mean serum Ghrelin levels are highest for2 weeks, the minimum is six weeks, showing the differences between the two have (P<0.05).2,4,6 weeks obese control group showed a mean serum Ghrelin levels gradually decline. Gastric tissue has the sametrend there is a significant difference (P<0.05) 2-week and 4 weeks and2weeks and 6 weeks have very show differences (P<0.01).8. Both the normal control group or stomach tissue Ghrelinserum levels and obesity in the control group average have the same variationin addition to two weeks and six weeks serum obese obesity gastric tissue than normal control group, the remaining obese control group than in the control group Ghrelin average content is relatively high. Conclusions:1. The content of obese rats Ghrelin body fat is relatively high at the beginning, with the body’s adaptation to the diet, Ghrelin levels decreased. Obesity is mainly associated with the consumption and energy consumption, this study found that the normal control group and the obese control group had the same variation, and mean serum levels of Ghrelin both gastric tissue was gradually extend downward trend over time. Dieting 3h group mean serum Ghrelin also have similar changes may be related to energy metabolism and food intake on the adaptability of the body. Aerobic exercise combined with eating causes weight loss control, energy metabolism in balance, there is conducive to good health.Ghrelin is a hunger hormone, obesity and food intake is closely related to uncontrolled food intake, increasing the body’s metabolism, body weight increases, reducing the release of Ghrelin, increased use of fat metabolism to lose weight, increase energy, regulate body energy metabolism in balance, so that the body is in dynamic equilibrium.2. The mean serum 3h resistance varies with the movement cycle extension Ghrelin was gradually rising trend change and dieting 3h average serum levels of Ghrelin Instead, explain to adapt with the exercise intensity, the balance of energy metabolism resistant rats, consumption increased food intake will not cause significant impact on the increase in body weight resistance group, not obesity resistant rats.But a diet ofrats with prolonged movement cycle, the body has to adapt to eating and exercise state control, reduce Ghrelin secretion may be mainly functional status of the body to adapt to the performance, in order to reduce food intake to lose weight. This study suggests that a combination ofaerobic exercise can effectively control food weight loss, weight loss purposes.3. Diet immediate group average levels of Ghrelin in gastric tissue is relatively small numerical difference between 4 and 6 weeks.But dieting instantly serum Ghrelin levels and exercise period is positively related to the movement of the stomach immediately after exercise are more affected by sympathetic innervation, subject to certain Ghrelin secretion inhibition, with adaptation to exercise, Ghrelin secretion adaptability increase, promote the release of growth hormone. |
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