Effects Of Aerobic And Acute Exercise On Leptin-AMPK In The Liver Of Type2Diabetic Rats

Objective:To develop an old male rat model of diet-induced obese and type II diabetic by high fat diets，and to study the effect of aerobic exercise and acute exercise on protein expression of leptinsensitivity and signaling in liver, this study tried to explore the mechanism that aerobic andacute exercise improves the disorder of leptin sensitivity and signaling in liver.Methods:1.52male rats11months old were randomly divided into4groups: normal control group，diabetes control group, aerobic campaign group, acute campaign group.12rats each group.The normal control group rats were feeded normal diet for16weeks. The groups exceptnormal control group were feeded high glucose and high fat diet for16weeks.2. Diabetic rat model were induced by intraperitoneal injection with strep to zotocin(STZ)atthe end of the twelveth week，then，aerobic campaign group were swimming trained eightweeks. Acute campaign group were swimming trained for one time before be killed in the24thweek.3.After16week-interventions,all the rats were killed and TG,FFA, hepatic glycogen,leptin,leptin receptors AMPKα1,p-AMPKα1,AMPKα2,p-AMPKα2,ACC and p-ACC inliver were determined.Results:1.Compared with normal control group, diabetes control group had significant increasedweight(P<0.01)，blood sugar(P<0.01)，insulin(P<0.01).Compared with normal control group,diabetes control group had increased TG(P<0.01)，FFA(P<0.05), hepatic glycogen(P<0.01) inliver. Compared with normal control group, diabetes control group had increased leptin(P<0.05),depressedAMPKα1(P<0.05),p-AMPKα1(P<0.05),AMPKα2(P<0.05),p-AMPKα2(P<0.01).Leptin receptor,ACC,P-ACC had not significant changed in liver.2.Compared with diabetes control group，aerobic campaign group had significant decreasedfasting blood glucose（P<0.01)and insulin (P<0.01). TG(P<0.01)，FFA(P<0.05), hepaticglycogen(P<0.05) had decreased significantly. Leptin（P<0.05）,ACC （P<0.05）in liverdecreased significantly. Compared with diabetes control group，aerobic campaign group had significant increased p-AMPKα1(P<0.05), AMPKα2(P<0.05), p-AMPKα2(P<0.05),p-ACC(P<0.05).3. Compared with diabetes control group，acute campaign group had significant decreasedinsulin (P<0.01). TG， FFA, hepatic glycogen had not decreased significantly. Leptin（P<0.05）,ACC （P<0.05）in liver decreased significantly. Compared with diabetes controlgroup， acute campaign group had significant increased p-AMPKα1(P<0.05),AMPKα2(P<0.01),p-AMPKα2(P<0.05).Body weight, fasting blood glucose,leptin receptor,AMPKα1,p-ACC had not changed a lot.Conclusion:1. Impaired leptin AMPK signal transduction pathway in type2diabetic rat liver is triggeredone of the mechanisms of hepatic glucose and lipid metabolism disorders.2. Chronic training can ease leptin resistance and improve glucose and lipid metabolismdisorders through the repair of liver leptin signal transduction pathway of AMPK liver leptinresistance.3. Acute exercise induced stress response of regulating glucose and lipid metabolism in liverby leptin AMPK signal transduction pathways sustained16hours.Suggestion:In the permission of the experiment condition,we can determine the density of MA to ensurethe coincidence of leptin signaling in the future.