Angelica Extract Synergies Inflammatory Compatibility Of Different Components, Anti-oxidation Studies

Objective:To investigate the anti-inflammatory angelica extract synergies of each component, antioxidant, explore the compatibility effect on the proportion of each component interactions, provide the basis for the study and application of Chinese angelica extractMethods:According to methods have been built, prepared the Supercritieal CO2extract of angelica sinensis, polysaccharides and total phenolic of Angelica, process optimization macroporous resin enrichment Angelica total phenolic acids; using LPS-induced RAW264.7cell to explore the synergistic anti-inflammatory effects between the Angelica Extracts, and between the main monomer components; and using DPPH method to explore the synergistic antioxidant effects between Angelica extracts, using AOM/DSS mouse colon cancer model to study angelica extract synergistic anti-inflammatory antioxidant effect. Isobologram analytical method and interaction index for analysis, to determine the compatibility of the proportion of each component Angelica synergyResults:Preferred Angelica acid extraction and separation processes:70%ethanol extract was concentrated to0.5g crude drug mL/-1,5000r.min-1, centrifugal30min, take the middle supernatant, the NKA-9macroporous resin. sample volume is9times the amount of column volume, flow rate of adsorption of0.5mL.min-1, a high ratio of diameter:1:8,3BV distilled water,3BV10%ethanol impurity, impurity flow rate of1mL.min-1,8BV45%ethanol elution flow rate:SmL.min-1, stable and feasible. Angelic the main component of ligustilide butylidenephthalide in compatibility with the ratio of1:10,1:20, significantly inhibited LPS-induced NO production in RAW264.7cell model, the ratio of two different concentrations of the mixture under the mutual the role of index τ is less than1, showed synergistic anti-inflammatory effects; in compatibility ratio of1:10, τ small compared with the average of1:20, the synergistic anti-inflammatory effects of the strongest under the scale. Angelica oil is the main component of ligustilide and Angelica total phenolic ferulic acid, the main ingredient in the next compatibility proportion1:10,1:20,1:30were able to significantly inhibit the performance ratio of the compatibility of synergistic anti-inflammatory effect, in which the ratio of compatibility1:30, τ small compared with the average of1:20, the synergistic anti-inflammatory effects of the strongest under the scale. Supercritieal CO2extract of angelica sinensis and Angelica total phenolic acids in raw herbs compatibility proportion when the ratio of1:2, the τ value of the minimum, the strongest synergy.Combination of total oil and total phenolic or polysaccharide can collaborately scavenge DPPH, exhibit synergistic antioxidant effect when the combination with high dose. Colon cancer model shows that Angelica polysaccharides and Supercritical CO2extract of angelica sinensis application compatibility effect on AOM/DSS-induced mice, the tumor inhibition rate lower than with Angelica Angelica polysaccharide or the total oil, their combination has a synergistic effect; relative to the model control group, but also to reduce the expression of s PCNA, COX-2and iNOS in colon.Conclusions:The main monomers in the extracs having a certain ratio relationship, angelica extracts also have a certain proportion of compatibility. Under combined in certain ratio, the compatibility of the components or monomers can produce a synergistic effect. This study can provides some proofs of the multi-level application of traditional medice, especially for the application of Angelica.