| Background: Unstable Angina is easily to turn to myocardial infarction in clinical. It can seriously affecting, and even endanger, the lives of patients. Studies have shown toll-like receptor signal transduction pathway may mediate immune and inflammatory reactions of unstable angina, with its level of its expression in cardiomyocytes, endothelial cells, etc. increased. Toll-like receptor involved in the formation of coronary atherosclerotic plaque and thrombus, and promote the development and development of the disease.Objective:By studying Tanshinone ⅡA Sulfonate Injection on Unstable Angina patients with toll-like receptor2, toll-like receptor4, myeloid differentiation factor88and nuclear factor KappaB effect, Evaluation by injecting Tanshinone ⅡA sulfonate in the treatment of coronary heart disease with unstable angina clinical efficacy in patients with blood stasis, to prove that the blood circulation medication unstable angina patients with blood stasis, the toll-like receptor signal transduction pathway level did reveals the purpose of traditional Chinese medicine by inhibiting Inflammation and in the treatment of angina pectoris.Methods:180cases of coronary heart disease of unstable angina patient from the GAMH cardiology department during Aug.2013and Mar.2014were selectd as group UA.20cases of healthy people from Southern District of the GAMH as control group. Using flow cytometry to compare and determine the two sets of the expression levels of toll-like receptor2, toll-like receptor4, myeloid differentiation factor88and nuclear factor KappaB in peripheral blood CD14+mononuclear cells between two groups.2Study select60patients with coronary heart disease with blood stasis syndrome, and20cases of coronary heart disease patients with non-blood stasis syndrome. Using flow cytometry to compare and determine the two sets of the expression levels of toll-like receptor2, toll-like receptor4, myeloid differentiation factor88and nuclear factor KappaB in peripheral blood CD14+mononuclear cells.360patients with coronary heart disease and blood stasis syndrome were randomly divided into two groups:control and treatment group. The control group were treated with western medicine. The treatment group treat in the same way as control group, but additionally treated by Tanshinone ⅡA sulfonate injection for10±2days. Before and after the treatment, study use cytometry to compare and determine the expression levels of toll-like receptor2, toll-like receptor4, myeloid differentiation factor88and nuclear factor KappaB in peripheral blood CD14+mononuclear cells in these two sets.4All data were analyzed and processed using SPSS19.0statistical software. Measurement data were presented as mean±standard deviation (x±s), count data using the frequency representation. The group before and after treatment using paired t test, measurement data between groups using independent sample t-test, count data between groups were compered with χ2test. P<0.05was statistically significant difference.Results:1Unstable angina patients’ expression levels of toll-like receptor2, toll-like receptor4, myeloid differentiation factor88and nuclear factor KappaB in peripheral blood CD14+mononuclear cells were significantly increased compared with healthy people.2Serum hs-CRP levels in unstable angina patients have positive correlation with peripheral blood CD14+monocytes toll-like receptor2, toll-like receptor4and nuclear factor KappaB protein expression levels.3Unstable angina patients with blood stasis syndrome peripheral blood CD14+monocytes toll-like receptor2, toll-like receptor4and nuclear factor KappaB protein expression levels and serum hs-CRP levels were significantly higher then coronary heart disease in patients with non-blood stasis syndrome, the difference was statistically significant.4Unstable angina patients with blood stasis syndrome peripheral blood CD14+monocytes myeloid differentiation factor88mean fluorescence intensity higher than coronary heart disease in patients with non-blood stasis syndrome, but the difference was not statistically significant.5Angina symptoms, ECG and blood stasis syndrome of TCM syndrome of treatment group were better then control group.6After treatment, toll-like receptor2, toll-like receptor4expression rate of treatment was lower than the control group, the difference was statistically significant, before and after treatment, compared toll-like receptor2, toll-like receptor4expression rate within each group, the difference was statistically significant.7After treatment, the difference of nuclear factor KappaB expression rate between two groups was not statistically significant, before and after treatment, compared nuclear factor KappaB expression rate within each group, the difference was statistically significant.8After treatment, myeloid differentiation factor88mean fluorescence intensity of treatment was lower than the control group, the difference was statistically significant, before and after treatment, compared nuclear factor KappaB expression rate within treatment group, the difference was statistically significant, the comparison within control group was not statistically significant.Conclusion:1The expression of unstable angina patients peripheral blood CD14+monocytes toll-like receptor2, toll-like receptor4, myeloid differentiation factor88and nuclear factor KappaB is increased.2Toll-like receptor2, toll-like receptor4are involved in the process of immune and inflammatory responses by MyD88-dependent pathway.3Unstable angina patients, which has blood stasis syndrome, serum hs-CRP levels were significantly higher then coronary heart disease in patients with non-blood stasis syndrome.4Serum hs-CRP levels in unstable angina patients have positive correlation with peripheral blood CD14+monocytes toll-like receptor2, toll-like receptor4and nuclear factor KappaB protein expression levels, they May play a synergistic role in disease development.5Unstable angina patients, which with blood stasis syndrome, their peripheral blood CD14+monocytes toll-like receptor2, toll-like receptor4and nuclear factor KappaB protein expression levels are significantly higher then coronary heart disease in patients with non-blood stasis syndrome.6Tanshinone ⅡA sulfonate injection in unstable angina treatment may inhibit TLR4/MyD88/NF-κB signaling pathway, by inhibiting the release of inflammatory cytokines in patients with UA, it can stable plaque and relieve angina symptoms. |
PDF Full Text Request