Chemical Constituents Of Peony Seeds

Dan pi is the mature and dried root bark of peony(Paeonia suffruticosa Andr.), in the "ShenNong’s Herbal Classic", it is first recorded, classified as medium grade drug, bitter, acrid; slightly cold, cold, is for liver, heart and kidney, removing the heatfrom blood, relieve pain, mainly used in the treatment of heat into blood, carbuncle swollen sore, warm spot poison, spitting blood, amenorrhea, dysmenorrhea.Peony seeds is the dried ripe seeds of peony (Paeonia suffruticosa Andr.), peony seeds has been used to treat leg pain in folk, it has antibacterial, antiinflammatory, antioxidant, analgesic effect, and the effect of certain medical care.Objective:In this paper, on the basis of previous studies, I summarized the research status of peony and peony seeds at home and abroad, the chemical constituents of the nonfat part of peony seeds, the HPLC determination of the content of the main active ingredient, the determination of crude protein content of peony seeds.Method:With gradient ethanol extraction method and using silica gel column chromatography, gel column chromatography, ODS column chromatography and the preparation of liquid chromatographic separation methods and means. According to the physical and chemical properties, spectra datas and synthetic analysis. Using Coomassie Brilliant Blue G-250staining and Bovine serum albumin as a reference,studied the determination of protein content of peony seeds, The second chapter has studied the determination of the main active ingredient content of peony seeds, paeoniflorin, albiflorin,6’-O-β-D-glucopyranosylalbiflorin, albiflorin Rl, trans-ε-viniferin, trans-resveratrol in peony seeds were determined by HPLC.Result:The first chapter has discussed about the extraction,separation and identification of the chemical constituents of Paeonia suffruticosa. seventeen constituents were separated from Paeonia suffruticosa, eleven constituents were from ethyl acetate parts, six constituents were from n-butanol parts. Fourteen constituents have been identified as follows:paeoniflorin, albiflorin,6’-O-β-D-glucopyranosylalbiflorin, albiflorin R1,p-hydroxybenzoic acid, oxy paeoniflo-rin, caffic acid, oleanolic acid, trans-ε-viniferin, trans-resveratrol, benzoic acid, sucrose,β-daucosterol,β-sitosteroL Among the compounds,p-hydroxybenzoic acid and caffic acid were isolated from the seeds of this plant for the first time, albiflorin R1was isolated from this plant for the first time.The second chapter has studied the determination of the main active ingredient content of peony seeds, paeoniflorin, albiflorin,6’-O-β-D-glucopyranosylalbiflorin, albiflorin R1, trans-ε-viniferin, trans-resveratrol in peony seeds were determined by HPLC. Paeoniflorin had a favourable linear relationship in the range of6.4-64μg.mL-1and the regression equation was as follows:Y=14894X+6516.9, r=0.9998(n=6); albiflorin had a favourable linear relationship in the range of6.2-62μg.mL-1and the regression equation was as follows:Y=11646X+8626.4, r=0.9998(n=6);6’-O-β-D-glucopyranosylalbiflorin had a favourable linear relationship in the range of7.2-72μg.mL-1and the regression equation was as follows: Y=10589X+549.57, r=0.9992(n=6); albiflorin R1had a favourable linear relationship in the range of1-10μg.mL-1and the regression equation was as follows:Y=16482X+9039.5, r=0.9993(n=6); trans-ε-viniferin had a favourable linear relationship in the range of5.2-52μg.mL-1and the regression equation was as follows:Y=15664X-40814, r=0.9992(n=6); trans-resveratrol had a favourable linear relationship in the range of0.17-2μg.mL-1and the regression equation was as follows:Y=11243X+3965.3, r=0.9993(n=6) the result of the main active ingredient content of peony seeds was as follows:albiflorin (0.21%),paeoniflorin(0.8%),6’-O-β-D-gluc-opyranosylalbiflorin (0.37%), albiflorin R1(0.08%), trans-ε-viniferin (0.44%), trans-resveratrol (0.001%), the content of paeoniflorin, albiflorin, trans-ε-viniferin in peony seeds was higher.The third chapter studied the determination of protein content of peony seeds, using Coomassie Brilliant Blue G-250staining and Bovine serum albumin as a reference, the content of soluble protein peony seeds meal were determined. The protein content of peony seeds is26.85%.The result showed that the protein content of peony seeds meal is higher, its nutritional value and safety need to be further studied, which provides a theoretical basis for the development and utilization of peony resources.Conclusion:1p-hydroxybenzoic acid and caffic acid were isolated from peony seeds for the fi rst time, albiflorin R1was isolated from this plant for the first time.2Simultaneous determination of paeoniflorin, albiflorin,6’-O-β-D-glucopyranosylalb iflorin, albiflorin R1, trans-ε-viniferin, trans-resveratrol, in peony seeds by high performa nce liquid chromatography(HPLC). It laid the foundation for the further research toimpr ove the quality control of peony seeds and provided a theoretical basis for the develop ment of peony seeds.3The protein content of peony seeds is26.85%.The result showed that the protein content of peony seeds meal is higher, its nutritional value and safety need to be further studied, which provides a theoretical basis for the development and utilization of peony resources.