Cordyceps militaris is a traditional edible and medicinal fungi, has high medicinal value, which is rich in bioactive substances and significant pharmacological effects had been received extensive attention of scholars both at home and abroad. Cordyceps militaris which contains cordyceps polysaccharide, cordycepin, cordyceps acid, SOD, sterols and other active ingredients, and contain a variety of protein, amino acids, vitamins and trace elements. A series of research result indicates that the cordyceps militaris has down three tenors, enhance immunity, anti-fatigue, antioxidation, antivirus, etc, but also has the role of adjuvant treatment of cardiovascular disease, renal dysfunction, liver disease, lung cancer treatment. This article militaris polysaccharide as the research object, in-depth study of its extraction process, a comprehensive analysis of its antioxidant activity, a preliminary study and analysis militaris complex polysaccharide synergistic antioxidant effect.(1)Cordyceps militaris polysaccharide extraction method and extraction conditions depth optimized to get the best cordyceps militaris polysaccharide extraction method for ultrasonic extraction, the optimum parameters for the93%of the ultrasonic power, ultrasonic time40min, ultrasonic temperature50℃, the ratio of waterl:40. The yield was15.48%under optimized conditions.(2) Cordyceps militaris polysaccharide after separation and purification, carries on the comprehensive analysis of the antioxidant activity in vitro. Polysaccharide of cordyceps militaris on DPPH and H2O2scavenging ability is stronger, its hydroxyl radical scavenging ability and superoxide anion scavenging ability general, the reduction ability is poorer.(3) Cordyceps militaris polysaccharide with small oyster mushrooms, agaricus bisporus, yellow umbrella and so on several common edible and medicinal fungi polysaccharide in different mixed ratio and concentration under the two composite. By two factor analysis of variance, cordyceps militaris polysaccharides and yellow umbrella composite effect is good, the composite ratio of2:1composite effect is best and present a good dose-effect relationship, the clearance rate compared with cordyceps militaris polysaccharide increased by44.39%, the yellow umbrella polysaccharide increased by71.93%. Cordyceps militaris polysaccharides with small oyster mushrooms polysaccharide compound effect is general, but also showed a certain degree of synergy. Cordyceps militaris polysaccharides with agaricus bisporus polysaccharide compound did not show a synergistic effect.(4) Cordyceps militaris polysaccharide with several polysaccharide composite test by orthogonal test principles and analytical methods. By analysis of variance, the clearance rate after three and four of the polysaccharide complex is improved to a certain extent, and showed a synergistic effect. |