| Objective：To observe the effect of Chinese herbal compound（CongGuiYiShen Capsule）improve clinical symptoms in patients with type2diabetic kidney disease in earlierperiod, the influence in laboratory indexes about glucose and renal function (such asfasting plasma glucose, postprandial2h plasma glucose, hemoglobin a1c, urea nitrogen,micro albuminuria）, and the serum levels of OPN、TGF-β1.At the same time, toobserve the protection of CongGuiYiShen Capsule on Type2DKD （Diabetic kidneydisease） rats and the influence of inflammation factor OPN、TGF-β1which related todiabetic kidney disease.Preliminary study on action link and the protection mechanismof Chinese herbal compound （CongGuiYiShen Capsule）to cure type2DKD from theperspective of Chinese medicine theory and inflammation cytokines, provide theclinical data and the theoretical basis for the TCM prevention and the treatment of DKDwith TCM method.Methods：1.clinical research：60patients of type2DKD in earlier period patients were randomlydivided into the treatment group and the control group, two groups were given basictreatment with western medicine（lotensin10mg oral qd）, the treatment group weretreated add Chinese herbal compound（CongGuiYiShen Capsule）. Respectively record theTCM syndromes and the laboratory indexes （FPG、2hPG、HbA1c、BUN、Scr、mALBand ACR）before and after the treatment, detect the changes of serum levels of OPN andTGF-β1, and comparing the differences between the two groups after treatment.2.Animal experiments：70male SD rats were randomly selected10rats as sham operationgroup which were fed with low fat diet, other rats were fed with high fat feed. After eightweeks, sutured the incision of sham operation group rats after incising the skin andmuscle layer. The rest60rats intraperitoneal were injected with STZ after the unilateralkidney ligation operation, to induce type2DKD in earlier period rats model. The model rats were randomly divided into three groups: model group、low dose group and highdose group. The sham operation group and the model group were given distilled waterby garage, low dose group and high dose group were given Chinese herbal compound（CongGuiYiShen Capsule）by garage with different dose. Continuously for12weeks,to observe type2diabetic kidney disease rats in general situation and laboratory indexes（glucose, hemoglobin A1c,24h urine volume, serum creatinine and blood urea nitrogen,24h urine protein quota）, test the level of serum OPN、TGF-β1,take out the kidneytissue used for testing the genetic expression levels of OPNmRNA.Results：1. Clinical Study：Clinical observation show: After treatment, the treatment group total efficiency was76.9%, the control group was53.6%. Compared with the two groups, the TCMsyndrome of the treatment group was superior to the control group （P＜0.05）,andsignificantly superior to pretherapy in the same group（P＜0.01）. The FPG、2hPG、HbA1c of the treatment group was lower than pretherapy（P＜0.01）, and better thancontrol group （P＜0.05）; The BUN、Scr、mALB、ACR of the treatment group andcontrol group were lower than pretherapy（P＜0.01）, and better than control group （P＜0.05or P＜0.01）,the treatment group was better than control group（P＜0.05）; TheOPN、TGF-β1of the treatment group and control group were lower than pretherapy（P＜0.01）, and better than control group（P＜0.05or P＜0.01）, the treatment group wasbetter than control group （P＜0.05or P＜0.01）.2. Animal experiments：Animal experimental results show: After modeling, compared with the sham operationgroup, Glu、HbA1c、Scr、Bun、24hurv of the rats which were modeled, have significantdifference（P＜0.01）。 After therapy, weight of TCM high and low dose group wasobviously higher compared with the model group（P＜0.01）,Glu、HbA1c levels werereduced（P＜0.01）。After therapy, Scr、Bun、mALB of the TCM high and low dosegroup were lower than the model group（P＜0.01）, the OPN、TGF-β1of high and low dose group were lower than the model control group （P <0.01）, OPNmRNAexpression of high and low dose groups was lower than the model control way of rats（P <0.01）.Conclusions：1. CongGuiYiShen Capsule can improve the TCM syndrome of the patients with Type2DKD in earlier period. It can reduce the BUN、Scr、mALB、ACR levels and cure Type2DKD in earlier period.2. CongGuiYiShen Capsule has the curative effect of preventing and curing DKD byreducing the serum of TGF-β1and OPNmRNA expression.3.CongGuiYiShen Capsule can effectively reduce the Glu、HbA1c、Scr、Bun、24h urv、24h upq levels of Type2DKD, improve the kidney function of Type2DKD rats whichinduced by STZ, have the function of preventing and curing of DKD.4. The levels of OPN、TGF-β1in Type2DKD rats were significantly higher than thesham operation group, which has showed model group rats indeed in a state of chronicinflammation, OPN and TGF-β1may be involved in the pathogenesis of DKD.5. The mechanism of CongGuiYiShen Capsule maybe achieved by reducing glucose,decreasing the excretion of urine protein, fighing inflammation, reducing the release ofinflammatory cytokines and the levels of OPN and TGF-β1. |
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