| Objective:To observe the intervention effect of Chinese herbal compound Chinese herbal compound Chinese herbal compound Chinese herbal compound( Cong Gui Yi Shen Capsule) improve clinical symptoms in patients with type 2diabetic kidney disease(DKD)CD68, MCP- 1 mrna expression level of influence. From the point of view to explore the role of inflammation and protection mechanisms about Cong Gui Yi Shen Capsule normalization with type 2 diabetes and kidney capsule of kidney disease(DKD) efficacy,to provide clinical data and theoretical basis for preventing and treating diabetic kidney disease with traditional Chinese medicine.Methods:1.clinical research : 60 patients of type 2 DKD earlier period with Kidney deficiency and blood stasis syndromein were randomly divided into the treatment group and the control group, The two groups were given the diet, blood glucose, blood pressure control and oral benazepril(10mg, qd) for the foundation treatment scheme,the treatment group were treated add Chinese herbal compound Cong Gui Yi Shen Capsule(0.4g/ particle,each time 5,3 times a day).Respectively record the patients with kidney deficiency and blood stasissyndrome integral, renal function, blood glucose level and other indicators before and after the treatment; detected the changes of serum levels of MCP-1 patients,The course of the treatment was 8 weeks.2.Animal experiments:70 male SD rats were randomly selected 10 as sham operation group which feed with low fat feed, other rats fed with high fat feed. Feeding time lasted for 8 weeks, sutured the incision of sham operation group rats after incising the skin and muscle layer. The rest 60 rats intraperitoneal were injected with STZ after the unilateral kidney ligation operation, to induce type 2 DKD in earlier period rats model. The model rats were randomly divided into three groups: model group、lowdose group and high dose group. The sham operation group and the model group were given distilled water by garage, low dose group and high dose group were given Chinese herbal compound(Cong Gui Yi Shen Capsule)by garage with different dose. To the end of the experiment the sham operation group, model control group, the number of Chinese medicine low dose group, high dose of Chinese medicine group rats were: 8,13, 13, 12. Observation of the rats in each group after 8 weeks(general behavior, body weight), and laboratory indexes(glucose, hemoglobin A1 c, 24 h urine volume, serum creatinine and blood urea nitrogen, 24 h urine protein quota),Detection of CD68, MCP-1 level in renal tissue, leave taking, weighing, to detect the expression of CD68, MPC-1m RNA by RT-PCR method.Results:1 Clinical Study:Clinical observation show: After 8 weeks,treatment, the treatment group compared with the control group, the symptom score than the control group(P <0.01), with the same group improved compared with before treatment significantly(P<0.01).Treatment group and the control group, the total effective rate was 81.5% and60.7%, respectively; after treatment, fasting blood glucose treatment group and the control group, 2h postprandial blood glucose, glycosylated hemoglobin compared with before treatment were significantly lower(P <0.05 or P <0.01), after treatmen the treatment group than the control group improvement(P <0.05)t; serum creatinine,urine albumin, blood urea nitrogen, creatinine and urine albumin than before treatment were decreased compared(P<0.05 or P<0.01)after treatment;compared with the control group, the treatment group improved significantly(P<0.05); the treatment group and the control group, serum MCP-1 levels were better than before treatment(P<0.01), and the treatment group was better than control group(P <0.01).2 Animal experiments:Animal experimental results show: After modeling, compared with the sham operation group, Glu、Hb A1c、Scr、Bun、24hurv of the rats which were modeled, have significantdifference(P<0.05 or P<0.01)。After therapy, TCM high and low dose group compared with the model group, weight was obviously higher(P<0.01),Glu、Hb A1 c levels were reduced(P<0.01)。After therapy, Scr、Bun、m ALB of the TCM high and low dose group was lower than the model group(P<0.05 or P<0.01), the MCP-1 of high and low dose group was lower than the model control group(P < 0.01).Conclusions:Cong Gui Yi Shen Capsule can effectively improve TCM syndrome integral and can effectively reduce the blood glucose and glycosylated hemoglobin, reduce the related indexes of renal function and MCP-1, CD68 gene expression level. The level of its mechanism might be related to the down-regulation of inflammatory cytokines,inflammatory reaction, improve insulin sensitivity, downregulation of MCP-1, the expression level of CD68 m RNA and the effective protect the kidney function related. |
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