The Influence Of Xinan Guben Peiyuan Recipe On Cytokines In Spleen Defficient Ulcerative Colitis In Rats

Objective: This study through the establishment of spleen deficient type ulcerativecolitis rats model, build up xinan guben peiyuan recipe to observe the effects ofcytokines on UC rats and explore its possible mechanism.Metheods: Will be60model rats were randomly divided into blank control groupSASP group (positive control)、 high dose group of guben peiyuan recipe dose、middle dose group of guben peiyuan recipe、low dose groupof gubenpeiyuan recipe；gives ordinary feeding blank control group, low dose group of guben peiyuan recipedose、middle dose group of guben peiyuan recipe dose、high dose group of gubenpeiyuan recipe dose、model group、SASP group make mould with senna and ice aceticacid, SASP group to willow nitrogen sulfanilamide pyridine lavage，in the low dosegroup、middle dose group、hight dose groupwas given guben peiyuan recipe decoctionlavage to observe the pathological change, signs and symptoms of rats and detect theserum IL-6、IL-8、IL-10and the concentration of TNF–a.Results: Model group rats enema senna on the first day of the occurrence diarrhoea,defecate is squishy or half paste, around the anus fur uncleanness, water quantityincreased, sleepiness, lazy to move. Anus into ice acetic acid, the second day of eachgroup rats diet is significantly reduced, vertical hair, easy removal, some appearabdominal distension. Male rats scrotal prolapse, the presence of mucous purulent blood,angular, squinting, expression the symptom such as malaise, spontaneous activitydecreased significantly. Up high, medium and low group of local school party colonicmucosa damage, which the exterior expansion guben peiyuan recipe high dose group isnot obvious, not seen obviously ulcer and erosion, accidentally see healing of ulcer scar;Dose group of colon color slightly, accidentally visible scar after the ulcer healing; Lowdose group of visible light edema; SASP group of colonic mucosa has no obviouserosion surface, and some mucosa by mild hyperemia, edema. Microscopically, thriftschool party high dose group, middle dose group has no mucous glands deficiency andinflammatory cell infiltration. Low dose group of the colonic mucosa of basic repair is complete, a small amount of bleeding; Model group colonic mucosa surface havedifferent degree of fall off necrosis, a large number of inflammatory cells infiltration;SASP group a few fragmentary fracture of colonic mucosa, have more inflammatorycells infiltration. Groups of rats serum cytokine analysis, according to the results ofmodel group IL-6,IL-8,TNF-a serum concentrations were significantly elevated, IL-0concentration is reduced, and SASP group, the blank group,guben peiyuan recipe washigh, medium and low group, with statistical significance (p <0.01); SASP group、highdose group,middle dose group compared with statistical significance (p <0.01);highdose group of Chinese medicine, compared with middle dose group、low dose group，with statistical significance (p<0.05); SASP groupcompared with middle dose group、low dose group of guben peiyuan recipe, no statistical significance (p>0.05).Conclusion:1Xinan guben peiyuan recipe can effective repair damaged spleen deficient typeulcerative colitis model rats of the colonic mucosa.2This topic in the study, Xinan guben peiyuan recipe can effective vely increase theulcerative colitis rats model with spleen deficient cytokines IL-10, And effectiveinhibition of IL-6、IL-8、the expression of TNF-a,Thus reduce healing factor of colonicmucosa damage, Protect the colonic mucosa.