Correlation Between Herpesvirus And Periodontopahtic Bacteria In Retrograde Pulpitis

Objective To explore the relationship between herpesvirus and fiveperiodontopathic bacteria in retrograde pulpitis, and the risk factor of retrogradepulpitis, get preliminary understanding about the mechanism of interactionbetween herpesvirus and five periodontopathic bacteria; and provide a scientificbasis for clinical diagnosis and treatment of retrograde pulpitis and severeperiodontal lesion.Method According to the previous data about the detection result ofAggregatibacter actinomycetemcomitans(A.a.), Porphyromonas gingivalis(P.g.),Prevotella intermedia(P.i.), Tannerella forsythensis(T.f.), Treponemadenticola(T.d.), human cytomegalovirus(HCMV) and Epstein-Barrvirus-1(EBV-1) in pulp tissue and subgingival plaque of38retrograde pulpitissamples and in subgingival plaque of30periodontal healthy samples, the datawas sorted and then performed using SPSS13.0statistical package, and appliedthe Chi-squared test to analyze the detection rate of periodontopathic bacteriaafter stratified according to EBV-1/HCMV detection, and the detection rate ofherpesvirus with different numbers of bacterial kinds. The Spearman’s rankcorrelation coefficient analysis was used in correlation calculations.Mann-Whitney U test was used for comparisons of the proportion of periodontopathic bacteria after stratified according to EBV-1/HCMV detection.Single variable logistic regression analysis was used for odds ratio-based riskcalculation of retrograde pulpitis.Result1. In the pulp tissue sample of retrograde pulpitis, the detectionrate of P.g. and T.f. with EBV-1positive was higher than the detection rate withEBV-1negative. The proportion of T.f. to the total bacteria was higher than theproportion with EBV-1negative in the subgingival plaque sample of retrogradepulpitis. A positive correlation was observed between EBV-1and T.f., the oddratio was1.10.2. In the pulp tissue sample of retrograde pulpitis, the proportion of P.i.、T.f.、T.d. to the total bacteria with HCMV positive was higher than theproportion with HCMV negative, and the proportion of T.f is obvious differentbetween two groups(P=0.049<0.05). In the subgingival plaque sample ofretrograde pulpitis, the proportion of P.g.、T.f. to the total bacteria with HCMVpositive was higher than the proportion with HCMV negative,A positivecorrelation was observed between HCMV and T.f., the odd ratio was1.06.3. The positive rate of EBV-1with3-5kinds of bacteria dectected inpulp tissue was significantly higher than those with no bacteriadetected(x2=5.657,P=0.043<0.05), and in herpesvirus positive samples, theproportion of infecting3-5kinds of bacteria is higher than the proportion ofinfecting and less than2kinds of bacteria.4. The risk factors for retrograde pulpitis were P.g.、P.i.、T.f.、T.d.,EBV-1and HCMV, their odd ratio were3.95,1.37,1.14,4.09,3.40and7.28respectively.Conclusion The risk factors for retrograde pulpitis were P.g.、P.i.、T.f.、T.d., EBV-1and HCMV. There may be interaction between EBV-1, HCMV and periodontopathic bacteria, especially with P.g. and T.f. And co-infection of manykinds of bacteria may work as a union and interact with herpesvirus of gettinginvolved in the disease progression. Yet there may be other pathogen orcombined pathogens getting involved with pathogenic mechanism of retrogradepulpitis and having interaction with herpesvirus because of the correlationbetween the five periodontopathic bacteria and herpesvirus of EBV-1andHCMV is not strong enough.