The Detection Of Telomere Length And The Research Of Homology In Synchronous Primary Carcinomas Of The Uterine And Ovary

Objective:To analysis whether have the same telomere length and ER、PR、P53molecular expression, and evaluate whether have the same histologic origin between endometrioid carcinomas of uterine and ovary.Methods:All tissues from a total of16synchronous primary carcinomas,43cases uterine endometrioid carcinomas and10cases ovarian endometrioid carcinomas. Quantitative PCR was used to detect the telomere DNA relative expression levels in16cases synchronous carcinomas,and PV-6000two-step was used to detect the immune-phenotype of estrogen receptor(ER), progesterone receptor(PR)and P53in all of the tissues,respectively.Results:(1)16cases synchronous tumors limited to the uterus (3.601±1.497) and ovary (3.71±1.556) have the similar telomere length. There was no statistically significant difference(T=1.423,P=0.175).(2) In synchronous carcinomas, there were10patients(62.5%) had the same immunophenotype of ER, Which was higher than that of the6patients (37.5%) who had differentially ER expressed, It was no significant difference between two sites tumors (P=0.06); There were9patients(56.25%) had the same immunophenotype of progesterone receptor (PR), Which was higher than that of the7patients (43,75%) who had differentially PR expressed, there was no significant difference between two sites tumors (P=0.09). There were9patients(56.25%) had the same immunophenotype of P53,Which was higher than that of the7patients (43,75%) who had differentially P53expressed, there was no statistically significant difference between two sites tumors (P=0.36).(3) There was no statistically significant difference of ER in synchronous carcinomas and corresponding single tumor, For endometrial carcinomas, P=0.65, for ovarian carcinomas,P=0,53; The same results toPR, In endometrial carcinomas, P=0.48, In ovarian carcinomas,P=0.42; But it was different to P53,In endometrial carcinomas, P=0.03, In ovarian carcinomas,P=0.04.It has significant difference between two species tumors.Conclusion:(1)In synchronous primary endometrioid adenocarcinomas of the uterine and ovary, Which have the similar telomere length and molecular events about ER, PR and P53at two sites tissues,So we preliminary calculate ovarian endometrioid adenocarcinoma probably have the same histologic origin with uterine endometrioid adenocarcinoma. While further studies are needed to the preliminary findings.(2) ER,PR molecular events may be similar between endometrium and ovary in synchronous primary cancers with corresponding single tumors. Except P53,which have the different molecular events,So there may be have some difference between synchronous primary carcinomas and corresponding single tumors.