The Establishment Of Casting Method And The Study Of Structure Concerning Stereostructural Model Of Gastric Artery Of Pig

Objective:To develop the pig gastric artery three-dimensional model, further study the stereoscopic structure of gastric arteries, and provide methods for making the gastric arterial model of experimental animals.Methods:The healthy grown-up pig’s stomach, spleen, liver and other organs are chosen as experimental objects. Based on the research and transform of the traditional method of vascular cast, pig gastric arterial three-dimensional models are made, thus the stereoscopic structure of gastric arteries from different angles and different sides can be observed and researched.Results:1. The tube for perfusion is developed; the puzzle of vascular cast is resolved by two point intubation from abdominal aorta and left gastroepiploic artery, two-way whole perfusion, seal for corrosion in static form and wash by carving; a method for making stereostructural models of gastric arteries was established.2. The main source of blood supply of the pig stomach is the celiac artery. Three main branches of celiac artery including the hepatic artery, splenic artery and left gastric artery were partly distributed in the gastric arteries. The overall shape of gastric arterial three-dimensional moldel is corresponding to the form of the living pig stomach. It’s a hollow cystic structure. The arteries of lesser and greater gastric curvature constitute the skeleton structure of gastric arteries. Lesser gastric curvature is constituted by a pair of "crow claw", which is out radially distributed in the surface of the stomach. Greater gastric curvature is composed of terminal arch anastomosis by left gastroepiploic artery, while the right gastroepiploic artery is on the greater curvature side. The "crow claw" on the visceral surface and wall surface tallies with the contralateral branches near the central section of stomach long axis.3. Left gastric artery and right gastric artery extend relatively on the visceral surface and wall surface, forming the "crow hand" of the reticular formation. Then they radially send out some branches, which form "crow claw", namely the framework of lesser gastric curvature. Left gastroepiploic artery and right gastroepiploic artery anastomose each other and form arch anastomosis of the greater curvature on the greater curvature side. The arch sends out some approximate parallel branches along the edge of greater curvature on the visceral surface and wall surface, which constitute the framework of greater gastric curvature.4. Gastric arteries have bow or serpentine bending line characteristics from the trunk to the terminal branch. The main and branch of the greater curvature bend obviously, but the lesser curvature’s bending degree is not distinct. Left gastric artery and right gastric artery rare bend.5. There is general anastomosis between gastric arteries, mainly the tiny branches anastomosis. The trunk anastomosis is not frequently seen. The arteries of each source are connected together through tiny branches and trunk anastomosis. They make gastric arteries form complete arterial blood pipe network.6. The gastric mucosal fold’s arteries in concave of arch anastomosis of the greater curvature can be seen clearly. It is a funicular arterial blood pipe network which is undulate bending curved extension. Again the multiple narrow funicular arterial blood pipe network can be seen at the bottom of the stomach.Conclusion:1. The effective method for making stereostructural models of gastric arteries is established. The stereoscopic structure of gastric arteries and relationship of the artery vasculatures between stomach and adjacent organs could be clearly displayed in the model. It provides methods for studying stereostructure of human gastric vessel.2. The shape of the pig gastric artery three-dimensional model corresponds to the living pig stomach. The three-dimensional structure of pig gastric arteries corresponds to the function of the stomach.3. This study observes and researches the stereoscopic structure of pig gastric arteries from different angles and different sides through the method of vascular cast. It provides comparative anatomy basis for human gastric artery research, and lay the foundation for further studying the disease states of human stomach vascular lesions.