Development And Evaluation Of Human Based Artificial Stomach Digestion Simulation System

Based on 3D printing technology,this study designs and constructs a system for simulating gastric digestion of animals-artificial stomach,which is used to study the food(or food model)force and decomposition in the adult stomach.The system is based on the basic characteristics of gastric anatomy and pHysiological changes after intake of food.The main features of these bodies are transformed into a computer-controlled vitro machine model that simulates the dynamics of thestomach,the frequency,parameters of the gastric fluid secretion,and the peristaltic waves produced by the movement of the stomach in different situations.Compared with those of the human stomach,the physiological parameters of the artificial stomach show that the artificial stomach could simulate the digestion of food in the stomach of the human stomach:in vitro,which provide the oretical basis and technical support for the food industry nutrition and digestion in vitro experiments.The paper has carried on the system research in the following several aspects:The human stomach model is constructed in full digitalization by using the water-soluble material and the 3D printing technique.On this basis,the artificial gastric digestive system is designed and constructied.The system consists mainly of the following structure:mechanical transmission system,electric compression system,gastric juice secretion and injection system,temperature control system and computer control system.Through continuous improvement and optimization of the key parameters of artificial stomach,such as gastric juice secretion rate,the role of the wall in the compressive force of the size,the system is closer to the real stomach of the dynamic gastric digestive process,including the mechanical transmission system for the movement of artificial stomach to provide power,electric compression device to simulate the stomach peristalsis,through a regular squeeze artificial stomach wall,both sides of the sensor measured the pressure range of 2.52 ± 0.15N and 3.32±0.16N,and 3 times per minute peristaltic circulation,the secretion of gastric juice and food samples are controlled by a computer-controlled syringe pump and peristaltic pump according to a specific program,the temperature control system can make the whole model at 37 ?constant temperature under conditions.In order to explore the real force bearing magnitude of food in the process of gastric digestion and quantitatively reproduce the body's human stomach grinding force,we designed and customized a spHerical aluminum mold with a diameter of 1.27 cm,using agar and guar gum as raw materials to make a gel pellet with a crushing force of 0.65N,which a hardness gradient was set up and down,to carry out experiments of the pellets in high viscosity and low viscosity food system in artificial stomach.We counted the number of complete pellets remaining in the stomach at each time point,obtained the relationship between the number of intact pellets remaining in the stomach and the digestion time for each experiment,and analyzed the multivariate function variance for the repeated measurements.From the number of rupture of the ball and the average rupture time,we can speculate that the actual force in the artificial stomach to confirm the body's human gastric grinding force can be simulated with artificial stomach.The experimental results show that the data measured in the artificial stomach are significantly correlated with the observed data in the human volunteers reported in the literature.The force of the artificial stomach through the extruded stomach wall is 0.2N?0.95N In the range.In this paper,rice is chosen as digested object,and the digestive process of rice is observed and studied in real time.The experiment mainly focuses on the real-time analysis of the disintegration and interaction between rice grains cooked in simulating human gastric motility.The main characteristics of the food components(including the typical grain size image after digestion,solid content,average particle size,etc.)and the pHysical properties of the gastric electrolyte solution(including kinematic viscosity,shear viscosity)are studied in the digestion process to provide the dynamic information of rice digestion in artificial stomachs and determine the superiority of artificial stomachs in the digestion process(especially solid food)compared with the existing in vitro digestive models,and further confirm that artificial stomachs can simulate the shearing force of food in true human stomach during the digestion.In summary,this paper designss and constructs a systematic artificial stomach in vitro to simulate human stomach digestion system,and through the quantitative control of artificial stomach compression and compression frequency,programmed to control the secretion and temperature of gastric juice,basically reproduced thereal stomach digestion in the process of dynamic conditions.Secondly,with the gel pellet simulation digestion confirmed the body's human stomach grinding force can be simulated with artificial stomach.Finally,through the real food rice digestion quantitative analysis,further evaluated artificial stomach in the simulation positive action in gastric digestion.