Procalcitonin Expression Changes And Clinical Research In Severe Acute Pancreatitis With Infection

Objective: To investigate the procalcitonin (procalcitonin, PCT) dynamic expressionand clinical research value in severe acute pancreatitis (SAP) in co-infection.Methods:100SD rats were randomly divided into four groups, sham operationgroup (Control group), acute infection group (LPS group), severe acute pancreatitis(SAP group), infection with severe acute pancreatitis (SAP+LPS group). After themodel is successful, each group in the postoperative serum PCT concentrations6,12,24,36,48h enzyme-linked immunosorbent assay (ELISA); ELISA assay ofserum IL-6, TNF-α expression; chelating reagent assay endotoxin levels in all rats;immunohistochemical staining was observed in rat pancreatic pathological changes.Results: LPS group, SAP group and SAP+LPS group than in the control groupincreased PCT from surgery6h,24h reached a peak,48h expression remains high,the gap was statistically significant (P <0.05); and SAP+LPS serum PCT comparedwith LPS group and SAP group were all higher expression each time period, the gapwas statistically significant (P <0.05); after LPS group, SAP group and SAP+LPSgroup of IL-6, TNF-α, endotoxin expression have increased, and the expression ofserum PCT and the relevant. Conclusion: The severe acute pancreatitis incombination with infection was associated with inflammation and infection degree ofthe body. The increasing of serum level of PCT was linked to the release ofinflammatory mediators in vivo. The serum level of PCT could be used as a potentialmarker in the diagnosis of the severe acute pancreatitis, and it’s valuable for us toexplore its application in clinical diagnosis.