The Expression And Relationship Of Chemokine CXCL4、Macrophages In Colorectal Cancer

ObjectiveInvestigating the expression of Chemokine CXCL4, macrophages in thetissue of colorectal cancer, analyzing the interrelationship between them andexploring its correlation to the clinicopathologic features of colotectal cancer.Methods160colorectal cancer tissues were picked and divided into four groupsaccording to TNM stage(I、II、III、IV) as test groups, the samples of controlgroups were obtained from adjacent normal operation tissues. Real-time PCRand Western-Blot were performed to detect the concentration of CXCL4incolorectal cancer tissues and the samples of control groups.Immunohistochemistry method was performed to detect the expression anddistribution of CXCL4and macrophages and analyzing the relationshipbetween the two indicators and the clinic pathological characteristics ofcolorectal cancer.Results1. CXCL4can express in colorectal cancer tissues and normal colorectaltissues adjacent to cancer lesion, the rate of CXCL4protein expression in colorectal cancer tissues was considerably higher than that in normalcolorectal tissues adjacent to cancer lesion (p<0.05).The level expression ofCXCL4protein was relevant with tumor size, lymph node metastasis, TNMstage (p<0.05),but no significant differences with gender, age and histologicaldifferentiation (p>0.05).2. Macrophages can expression in colorectal cancer tissues and normalcolorectal tissues adjacent to cancer lesion. The rate of macrophages incolorectal cancers was considerably higher than that in normal colorectaltissues adjacent to cancer lesion (p<0.05). The level expression ofmacrophages was relevant with clinical stage, lymphatic metastasis incolorectal cancer (p<0.05).3. There was a positive correlation between CXCL4and macrophages incolorectal cancer tissues.Conclusions1. The expression of CXCL4was up-regulated in colorectal cancertissues, and the level of expression of CXCL4was correlated to theprogression and metastasis of colorectal cancer. 2. CXCL4could be produced by both tumor cells and macrophage, alsocould attract more macrophage to tumor site. CXCL4and macrophagesinfluenced synergistically the progression of colorectal cancer.